ArticleBiophysical journal2006
Mechanism and dynamics of breakage of fluorescent microtubules.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Microtubule lattice conformation and integrity regulate α-tubulin acetylation.Communications biology · 2026Article
- An antioxidant screen identifies ascorbic acid for prevention of light-induced mitotic prolongation in live cell imaging.Communications biology · 2023Article
- The rate of microtubule breaking increases exponentially with curvature.Scientific reports · 2022Article
- Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins.Journal of visualized experiments : JoVE · 2022Article
- Oxidative stress pathogenically remodels the cardiac myocyte cytoskeleton via structural alterations to the microtubule lattice.Developmental cell · 2021Article
- Article
- In Vitro Microtubule Dynamics Assays Using Dark-Field Microscopy.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Influence of GHz electric fields on the mechanical properties of a microtubule.Journal of molecular modeling · 2015Article
- External electric field effects on the mechanical properties of the αβ-tubulin dimer of microtubules: a molecular dynamics study.Journal of molecular modeling · 2014Article
- Viscoelasticity of tau proteins leads to strain rate-dependent breaking of microtubules during axonal stretch injury: predictions from a mathematical model.Biophysical journal · 2014Article
- An improved optical tweezers assay for measuring the force generation of single kinesin molecules.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Covalent immobilization of microtubules on glass surfaces for molecular motor force measurements and other single-molecule assays.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The breakage of fluorescence-labeled microtubules under irradiation of excitation light is found in our experiments. Its mechanism is studied. The results indicate that free radicals are the main reason for the photosensitive breakage. Furthermore, the mechanical properties of the microtubules are probed with a dual-optical tweezers system. It is found that the fluorescence-labeled microtubules are much easier to extend compared with those without fluorescence. Such microtubules can be extended by 30%, and the force for breaking them up is only several piconewtons. In addition, we find that the breakup of the protofilaments is not simultaneous but step-by-step, which further confirms that the interaction between protofilaments is fairly weak.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.