ArticleBiophysical journal2006
Local induction of acetylcholine receptor clustering in myotube cultures using microfluidic application of agrin.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 29 citations in OpenAlex.
- Principles for the design of multicellular engineered living systems.APL bioengineering · 2022Article
- Article
- "Chip-on-a-Transwell" Devices for User-Friendly Control of the Microenvironment of Cultured Cells.ACS applied bio materials · 2019Article
- The upcoming 3D-printing revolution in microfluidics.Lab on a chip · 2016Review
- An open-chamber flow-focusing device for focal stimulation of micropatterned cells.Biomicrofluidics · 2016Article
- Microtechnologies to fuel neurobiological research with nanometer precision.Journal of nanobiotechnology · 2013Review
- Extensive adipogenic and osteogenic differentiation of patterned human mesenchymal stem cells in a microfluidic device.Lab on a chip · 2010Article
- Cell adhesion to agrin presented as a nanopatterned substrate is consistent with an interaction with the extracellular matrix and not transmembrane adhesion molecules.BMC cell biology · 2008Article
- Localized acetylcholine receptor clustering dynamics in response to microfluidic focal stimulation with agrin.Biophysical journal · 2008Article
- A hybrid microfluidic-vacuum device for direct interfacing with conventional cell culture methods.BMC biotechnology · 2007Article
- Long-term microfluidic cultures of myotube microarrays for high-throughput focal stimulation.Nature protocols · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
During neuromuscular synaptogenesis, the exchange of spatially localized signals between nerve and muscle initiates the coordinated focal accumulation of the acetylcholine (ACh) release machinery and the ACh receptors (AChRs). One of the key first steps is the release of the proteoglycan agrin focalized at the axon tip, which induces the clustering of AChRs on the postsynaptic membrane at the neuromuscular junction. The lack of a suitable method for focal application of agrin in myotube cultures has limited the majority of in vitro studies to the application of agrin baths. We used a microfluidic device and surface microengineering to focally stimulate muscle cells with agrin at a small portion of their membrane and at a time and position chosen by the user. The device is used to verify the hypothesis that focal application of agrin to the muscle cell membrane induces local aggregation of AChRs in differentiated C2C12 myotubes.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.