ArticleBiophysical journal2006
Syntillas release Ca2+ at a site different from the microdomain where exocytosis occurs in mouse chromaffin cells.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Thirty years of CaBiophysics reports · 2024Article
- Alpha herpesvirus exocytosis from neuron cell bodies uses constitutive secretory mechanisms, and egress and spread from axons is independent of neuronal firing activity.PLoS pathogens · 2024Article
- SICT: automated detection and supervised inspection of fast CaScientific reports · 2018Article
- Catecholamine exocytosis during low frequency stimulation in mouse adrenal chromaffin cells is primarily asynchronous and controlled by the novel mechanism of Ca2+ syntilla suppression.The Journal of physiology · 2014Article
- Functional ryanodine receptors in the membranes of neurohypophysial secretory granules.The Journal of general physiology · 2014Article
- Ca2+ sparks and puffs are generated and interact in rat hippocampal CA1 pyramidal neuron dendrites.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- The cellular and molecular basis of bitter tastant-induced bronchodilation.PLoS biology · 2013Article
- Ryanodine Receptors Selectively Interact with L Type Calcium Channels in Mouse Taste Cells.PloS one · 2013Article
- Understanding calcium waves and sparks in central neurons.Nature reviews. Neuroscience · 2012Review
- Type 1 ryanodine receptor knock-in mutation causing central core disease of skeletal muscle also displays a neuronal phenotype.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Spatial organization of RYRs and BK channels underlying the activation of STOCs by Ca(2+) sparks in airway myocytes.The Journal of general physiology · 2011Article
- Minding the calcium store: Ryanodine receptor activation as a convergent mechanism of PCB toxicity.Pharmacology & therapeutics · 2010Review
- Individual calcium syntillas do not trigger spontaneous exocytosis from nerve terminals of the neurohypophysis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Suppression of Ca2+ syntillas increases spontaneous exocytosis in mouse adrenal chromaffin cells.The Journal of general physiology · 2009Article
- A close association of RyRs with highly dense clusters of Ca2+-activated Cl- channels underlies the activation of STICs by Ca2+ sparks in mouse airway smooth muscle.The Journal of general physiology · 2008Article
- Ca2+-sparks constitute elementary building blocks for global Ca2+-signals in myocytes of retinal arterioles.Cell calcium · 2007Article
- Ca(2+) sparks operated by membrane depolarization require isoform 3 ryanodine receptor channels in skeletal muscle.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Dihydropyridine receptors and type 1 ryanodine receptors constitute the molecular machinery for voltage-induced Ca2+ release in nerve terminals.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
Spontaneous, short-lived, focal cytosolic Ca2+ transients were found for the first time and characterized in freshly dissociated chromaffin cells from mouse. Produced by release of Ca2+ from intracellular stores and mediated by type 2 and perhaps type 3 ryanodine receptors (RyRs), these transients are quantitatively similar in magnitude and duration to Ca2+ syntillas in terminals of hypothalamic neurons, suggesting that Ca2+ syntillas are found in a variety of excitable, exocytotic cells. However, unlike hypothalamic nerve terminals, chromaffin cells do not display syntilla activation by depolarization of the plasma membrane, nor do they have type 1 RyRs. It is widely thought that focal Ca2+ transients cause "spontaneous" exocytosis, although there is no direct evidence for this view. Hence, we monitored catecholamine release amperometrically while simultaneously imaging Ca2+ syntillas, the first such simultaneous measurements. Syntillas failed to produce exocytotic events; and, conversely, spontaneous exocytotic events were not preceded by syntillas. Therefore, we suggest that a spontaneous syntilla, at least in chromaffin cells, releases Ca2+ into a cytosolic microdomain distinct from the microdomains containing docked, primed vesicles. Ryanodine (100 microM) reduced the frequency of Ca2+ syntillas by an order of magnitude but did not alter the frequency of spontaneous amperometric events, suggesting that syntillas are not involved in steps preparatory to spontaneous exocytosis. Surprisingly, ryanodine also increased the total charge of individual amperometric events by 27%, indicating that intracellular Ca2+ stores can regulate quantal size.
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