ArticleBiophysical journal2006
Differential stiffness and lipid mobility in the leaflets of purple membranes.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- A Minimalist Model Lipid System Mimicking the Biophysical Properties ofLangmuir : the ACS journal of surfaces and colloids · 2025Article
- Bacteriorhodopsin of purple membrane reverses anisotropy outside the pH range of proton pumping based on logic gate realization.Scientific reports · 2024Article
- Peptide-Coated Bacteriorhodopsin-Based Photoelectric Biosensor for Detecting Rheumatoid Arthritis.Biosensors · 2023Article
- Direct and Label-Free Determination of Human Glycated Hemoglobin Levels Using Bacteriorhodopsin as the Biosensor Transducer.Sensors (Basel, Switzerland) · 2020Article
- AFM nanoindentation reveals decrease of elastic modulus of lipid bilayers near freezing point of water.Scientific reports · 2019Article
- Versatile Protein-A Coated Photoelectric Immunosensors with a Purple-Membrane Monolayer Transducer Fabricated by Affinity-Immobilization on a Graphene-Oxide Complexed Linker and by Shear Flow.Sensors (Basel, Switzerland) · 2018Article
- Multifrequency AFM reveals lipid membrane mechanical properties and the effect of cholesterol in modulating viscoelasticity.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Ions Modulate Stress-Induced Nanotexture in Supported Fluid Lipid Bilayers.Biophysical journal · 2017Article
- Effect of dacarbazine on CD44 in live melanoma cells as measured by atomic force microscopy-based nanoscopy.International journal of nanomedicine · 2017Article
- Artesunate altered cellular mechanical properties leading to deregulation of cell proliferation and migration in esophageal squamous cell carcinoma.Oncology letters · 2015Article
- Origins of phase contrast in the atomic force microscope in liquids.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Thermal response of Langmuir-Blodgett films of dipalmitoylphosphatidylcholine studied by atomic force microscopy and force spectroscopy.Biophysical journal · 2007Article
- Electrostatic and steric interactions determine bacteriorhodopsin single-molecule biomechanics.Biophysical journal · 2007Article
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Purple membranes (PM) are two-dimensional crystals formed by bacteriorhodopsin and a variety of lipids. The lipid composition and density in the cytoplasmic (CP) leaflet differ from those of the extracellular (EC) leaflet. A new way of differentiating the two sides of such asymmetric membranes using the phase signal in alternate contact atomic force microscopy is presented. This method does not require molecular resolution and is applied to study the stiffness and intertrimer lipid mobility in both leaflets of the PM independently over a broad range of pH and salt concentrations. PM stiffens with increasing salt concentration according to two different regimes. At low salt concentration, the membrane Young's normal modulus grows quickly but differentially for the EC and CP leaflets. At higher salt concentration, both leaflets behave similarly and their stiffness converges toward the native environment value. Changes in pH do not affect PM stiffness; however, the crystal assembly is less pronounced at pH > or = 10. Lipid mobility is high in the CP leaflet, especially at low salt concentration, but negligible in the EC leaflet regardless of pH or salt concentration. An independent lipid mobility study by solid-state NMR confirms and quantifies the atomic force microscopy qualitative observations.
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