ArticleJournal of bacteriology2006
Genetic characterization of a single bifunctional enzyme for fumarate reduction and succinate oxidation in Geobacter sulfurreducens and engineering of fumarate reduction in Geobacter metallireducens.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 89 citations in OpenAlex.
- Overview of pathways and dynamic interactions of c-type cytochromes in Geobacter sulfurreducens.Biochemical Society transactions · 2026Review
- Structural basis of menaquinone reduction by succinate dehydrogenase from Chloroflexus aurantiacus.Nature communications · 2025Article
- Article
- Influence of support materials on the electroactive behavior, structure and gene expression of wild type and GSU1771-deficient mutant of Geobacter sulfurreducens biofilms.Environmental science and pollution research international · 2025Article
- Electron transport across the cell envelope via multihemeFrontiers in chemistry · 2025Review
- Anaerobic benzene oxidation inApplied and environmental microbiology · 2024Article
- Article
- Global transcriptional analysis of Geobacter sulfurreducens gsu1771 mutant biofilm grown on two different support structures.PloS one · 2023Article
- Geobacter sulfurreducens metabolism at different donor/acceptor ratios.MicrobiologyOpen · 2022Article
- Reduction Kinetic of Water Soluble Metal Salts byFrontiers in microbiology · 2022Article
- A New Approach for Evaluating Electron Transfer Dynamics by UsingApplied and environmental microbiology · 2020Article
- The hidden chemolithoautotrophic metabolism of Geobacter sulfurreducens uncovered by adaptation to formate.The ISME journal · 2020Article
- Article
- Enhanced Growth of Pilin-Deficient Geobacter sulfurreducens Mutants in Carbon Poor and Electron Donor Limiting Conditions.Microbial ecology · 2019Article
- Characterization of the genome fromRSC advances · 2019Article
- Characterization of a Novel Porin-Like Protein, ExtI, from Geobacter sulfurreducens and Its Implication in the Reduction of Selenite and Tellurite.International journal of molecular sciences · 2018Article
- Carboxydotrophic growth of Geobacter sulfurreducens.Applied microbiology and biotechnology · 2016Article
- Metabolic Profiling of Geobacter sulfurreducens during Industrial Bioprocess Scale-Up.Applied and environmental microbiology · 2015Article
- Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens.PLoS computational biology · 2014Article
- Bioremediation: a genuine technology to remediate radionuclides from the environment.Microbial biotechnology · 2013Review
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7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanism of fumarate reduction in Geobacter sulfurreducens was investigated. The genome contained genes encoding a heterotrimeric fumarate reductase, FrdCAB, with homology to the fumarate reductase of Wolinella succinogenes and the succinate dehydrogenase of Bacillus subtilis. Mutation of the putative catalytic subunit of the enzyme resulted in a strain that lacked fumarate reductase activity and was unable to grow with fumarate as the terminal electron acceptor. The mutant strain also lacked succinate dehydrogenase activity and did not grow with acetate as the electron donor and Fe(III) as the electron acceptor. The mutant strain could grow with acetate as the electron donor and Fe(III) as the electron acceptor if fumarate was provided to alleviate the need for succinate dehydrogenase activity in the tricarboxylic acid cycle. The growth rate of the mutant strain under these conditions was faster and the cell yields were higher than for wild type grown under conditions requiring succinate dehydrogenase activity, suggesting that the succinate dehydrogenase reaction consumes energy. An orthologous frdCAB operon was present in Geobacter metallireducens, which cannot grow with fumarate as the terminal electron acceptor. When a putative dicarboxylic acid transporter from G. sulfurreducens was expressed in G. metallireducens, growth with fumarate as the sole electron acceptor was possible. These results demonstrate that, unlike previously described organisms, G. sulfurreducens and possibly G. metallireducens use the same enzyme for both fumarate reduction and succinate oxidation in vivo.
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