ArticleProceedings of the National Academy of Sciences of the United States of America2006
Flow cytometric detection of specific RNAs in native human cells with quenched autoligating FRET probes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Toward Ideal Proximity-Driven Reactions in Chemical Biology.Chembiochem : a European journal of chemical biology · 2026Review
- A Modular Approach for the Synthesis of Diverse Heterobifunctional Cyanine Dyes.The Journal of organic chemistry · 2024Article
- Expanding the scope of native chemical ligation - templated small molecule drug synthesisChemical science · 2021Article
- DNA/RNA Fluorescence Imaging by Synthetic Nucleic Acids.Advances in experimental medicine and biology · 2021Article
- Comparing Agent-Based Delivery of DNA and PNA Forced Intercalation (FIT) Probes for Multicolor mRNA Imaging.Chembiochem : a European journal of chemical biology · 2019Article
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- A bright FIT-PNA hybridization probe for the hybridization state specific analysis of a C → U RNA editChemical science · 2018Article
- Chemical ligation of oligonucleotides using an electrophilic phosphorothioester.Nucleic acids research · 2017Article
- Nucleic Acid Templated Reactions for Chemical Biology.ChemMedChem · 2017Review
- Subnanomolar Detection of Oligonucleotides through Templated Fluorogenic Reaction in Hydrogels: Controlling Diffusion to Improve Sensitivity.Angewandte Chemie (International ed. in English) · 2017Article
- Live Cell Imaging of Endogenous mRNA Using RNA-Based Fluorescence "Turn-On" Probe.ACS chemical biology · 2017Article
- Nucleic Acid Templated Chemical Reaction in a Live Vertebrate.ACS central science · 2016Article
- The Non-Specific Binding of Fluorescent-Labeled MiRNAs on Cell Surface by Hydrophobic Interaction.PloS one · 2016Article
- Template-directed ligation on repetitive DNA sequences: a chemical method to probe the length of Huntington DNA.Chemical science · 2015Article
- Single molecule fluorescence approaches shed light on intracellular RNAs.Chemical reviews · 2014Review
- Fluorescent probes for nucleic Acid visualization in fixed and live cells.Molecules (Basel, Switzerland) · 2013Review
- Single cell spectroscopy: noninvasive measures of small-scale structure and function.Methods (San Diego, Calif.) · 2013Review
- Imaging mRNA expression levels in living cells with PNA·DNA binary FRET probes delivered by cationic shell-crosslinked nanoparticles.Organic & biomolecular chemistry · 2013Article
- Application of live-cell RNA imaging techniques to the study of retroviral RNA trafficking.Viruses · 2012Review
- Oligonucleotide-templated reactions for sensing nucleic acids.Molecules (Basel, Switzerland) · 2012Review
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2 authors.
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Abstract
We describe the use of modified fluorescent-labeled oligonucleotide probes in the sequence-specific detection of messenger RNAs in live human cells. To make this detection possible, we developed a previously undescribed probe design that combines earlier quenched autoligation chemistry with a previously undescribed fluorescence resonance energy transfer (FRET) strategy to lower background signals. The probe pairs consisted of a nucleophilic 3'-phosphorothioate probe carrying a Cy5 FRET acceptor, and an electrophilic probe containing the combination of a 5' end electrophile/quencher and a fluorescein FRET donor. Probes were introduced to HL-60 cells by use of the streptolysin O pore-forming peptide. Signals from three different messenger RNAs, as well as 28S ribosomal RNA, could be detected and quantitated by flow cytometry. Probes targeted to ribosomal sequences and beta-actin mRNA also could be detected over background by confocal fluorescence microscopy. Varying the target site and probe backbone chemistry were found to have large effects on signal. The data suggest that quenched autoligating probes may be of general utility as biological tools in following localization, transcription, and processing of eukaryotic cellular messages and may have applications in diagnostic or prognostic analysis of disease-related RNAs in human tissues.
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