ArticleMolecular biology of the cell2006
Cdc42 and actin control polarized expression of TI-VAMP vesicles to neuronal growth cones and their fusion with the plasma membrane.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 93 citations in OpenAlex.
- Review
- Assays of Platelet SNARE-actin Interactions.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Syntaxin-1/TI-VAMP SNAREs interact with Trk receptors and are required for neurotrophin-dependent outgrowth.Oncotarget · 2018Article
- Primary Cilia Reconsidered in the Context of Ciliopathies: Extraciliary and Ciliary Functions of Cilia Proteins Converge on a Polarity theme?BioEssays : news and reviews in molecular, cellular and developmental biology · 2018Review
- Plasma membrane LAT activation precedes vesicular recruitment defining two phases of early T-cell activation.Nature communications · 2018Article
- Spatiotemporal organization of exocytosis emerges during neuronal shape change.The Journal of cell biology · 2018Article
- Vamp-7-dependent secretion at the immune synapse regulates antigen extraction and presentation in B-lymphocytes.Molecular biology of the cell · 2017Article
- Beyond the cytoskeleton: The emerging role of organelles and membrane remodeling in the regulation of axon collateral branches.Developmental neurobiology · 2016Review
- FLIM FRET Visualization of Cdc42 Activation by Netrin-1 in Embryonic Spinal Commissural Neuron Growth Cones.PloS one · 2016Article
- Cdc42-dependent actin dynamics controls maturation and secretory activity of dendritic cells.The Journal of cell biology · 2015Article
- Article
- Platelet geometry sensing spatially regulates α-granule secretion to enable matrix self-deposition.Blood · 2015Article
- Molecular underpinnings of synaptic vesicle pool heterogeneity.Traffic (Copenhagen, Denmark) · 2015Review
- Neurite outgrowth induced by NGF or L1CAM via activation of the TrkA receptor is sustained also by the exocytosis of enlargeosomes.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Steering neuronal growth cones by shifting the imbalance between exocytosis and endocytosis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells.Molecular cancer · 2014Article
- Use of pHluorin to assess the dynamics of axon guidance receptors in cell culture and in the chick embryo.Journal of visualized experiments : JoVE · 2014Article
- Rho GTPases, phosphoinositides, and actin: a tripartite framework for efficient vesicular trafficking.Small GTPases · 2014Review
- Molecular complexes that direct rhodopsin transport to primary cilia.Progress in retinal and eye research · 2014Review
- Platelet granule exocytosis: a comparison with chromaffin cells.Frontiers in endocrinology · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tetanus neurotoxin-insensitive vesicle-associated membrane protein (TI-VAMP)-mediated fusion of intracellular vesicles with the plasma membrane is crucial for neurite outgrowth, a pathway not requiring synaptobrevin-dependent exocytosis. Yet, it is not known how the TI-VAMP membrane trafficking pathway is regulated or how it is coordinated with cytoskeletal dynamics within the growth cone that guide neurite outgrowth. Here, we demonstrate that TI-VAMP, but not synaptobrevin 2, concentrates in the peripheral, F-actin-rich region of the growth cones of hippocampal neurons in primary culture. Its accumulation correlates with and depends upon the presence of F-actin. Moreover, acute stimulation of actin remodeling by homophilic activation of the adhesion molecule L1 induces a site-directed, actin-dependent recruitment of the TI-VAMP compartment. Expression of a dominant-positive mutant of Cdc42, a key regulator of cell polarity, stimulates formation of F-actin- and TI-VAMP-rich filopodia outside the growth cone. Furthermore, we report that Cdc42 activates exocytosis of pHLuorin tagged TI-VAMP in an actin-dependent manner. Collectively, our data suggest that Cdc42 and regulated assembly of the F-actin network control the accumulation and exocytosis of TI-VAMP-containing membrane vesicles in growth cones to coordinate membrane trafficking and actin remodeling during neurite outgrowth.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.