ArticleJournal of virology2006
Point mutations upstream of hepatitis B virus core gene affect DNA replication at the step of core protein expression.
Article in Journal of virology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 52 citations in OpenAlex.
- Impact of a naturally occurring hepatitis B virus genotype D-specific core-promoter mutation on viral replication.The Journal of general virology · 2026Article
- Molecular analysis of HBV pre-core gene mutations in patients co-infected with HIV at a tertiary care hospital in North India.Access microbiology · 2025Article
- In vitro expression of precore proteins of hepatitis B virus subgenotype A1 is affected by HBcAg, and can affect HBsAg secretion.Scientific reports · 2021Article
- Article
- Case Report: Application of hepatitis B virus (HBV) deep sequencing to distinguish between acute and chronic infection.Wellcome open research · 2020Article
- Article
- LUC7L3/CROP inhibits replication of hepatitis B virus via suppressing enhancer II/basal core promoter activity.Scientific reports · 2016Article
- Functional characterization of hepatitis B virus core promoter mutants revealed transcriptional interference among co-terminal viral mRNAs.The Journal of general virology · 2016Article
- Precore/core region mutations of hepatitis B virus related to clinical severity.World journal of gastroenterology · 2016Review
- Correlation Between Hepatitis B G1896A Precore Mutations and HBeAg in Chronic HBV Patients.Jundishapur journal of microbiology · 2015Article
- Hepatitis B virus genotype distribution and genotype-specific BCP/preCore substitutions in acute and chronic infections in Argentina.PloS one · 2015Article
- Quasispecies structure, cornerstone of hepatitis B virus infection: mass sequencing approach.World journal of gastroenterology · 2013Review
- Short hairpin RNAs with a 2- or 3-base mismatch inhibit HBV expression and replication in HepG2 cells.Hepatology international · 2013Article
- Effects of hepatitis B virus mutations on its replication and liver disease severity.The open virology journal · 2013Article
- Ultra-deep pyrosequencing analysis of the hepatitis B virus preCore region and main catalytic motif of the viral polymerase in the same viral genome.Nucleic acids research · 2011Article
- Hepatitis B virus genotype C isolates with wild-type core promoter sequence replicate less efficiently than genotype B isolates but possess higher virion secretion capacity.Journal of virology · 2011Article
- Characterization of occult hepatitis B virus infection from blood donors in China.Journal of clinical microbiology · 2011Article
- Improved method for rapid and efficient determination of genome replication and protein expression of clinical hepatitis B virus isolates.Journal of clinical microbiology · 2011Article
- APOBEC3G directly binds Hepatitis B virus core protein in cell and cell free systems.Virus research · 2010Article
- Massive APOBEC3 editing of hepatitis B viral DNA in cirrhosis.PLoS pathogens · 2010Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The pregenomic RNA directs replication of the hepatitis B virus (HBV) genome by serving both as the messenger for core protein and polymerase and as the genome precursor following its packaging into the core particle. RNA packaging is mediated by a stem-loop structure present at its 5' end designated the epsilon signal, which includes the core gene initiator AUG. The precore RNA has a slightly extended 5' end to cover the entire precore region and, consequently, directs the translation of a precore/core protein, which is secreted as e antigen (HBeAg) following removal of precore-derived signal peptide and the carboxyl terminus. A naturally occurring G1862T mutation upstream of the core AUG affects the bulge of the epsilon signal and generates a "forbidden" residue at the -3 position of the signal peptide cleavage site. Transfection of this and other mutants into human hepatoma cells failed to prove their inhibition of HBeAg secretion but rather revealed great impairment of genome replication. This replication defect was associated with reduced expression of core protein and could be overcome by a G1899A covariation, or by nonsense or frameshift mutation in the precore region. All these mutations antagonized the G1862T mutation on core protein expression. Cotransfection of the G1862T mutant with a replication-deficient HBV genome that provides core protein in trans also restored genome replication. Consistent with our findings in cell culture, HBV genotype A found in African/Asian patients has T1862 and is associated with much lower viremia titers than the European subgroup of genotype A.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.