ArticleNucleic acids research2005
Solution structure of psi32-modified anticodon stem-loop of Escherichia coli tRNAPhe.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Structural Impact of 3-methylcytosine Modification on the Anticodon Stem-loop of a Neuronally-enriched Arginine tRNA.Journal of molecular biology · 2025Article
- The role of RNA modification in hepatocellular carcinoma.Frontiers in pharmacology · 2022Review
- Mining for Perchlorate Resistance Genes in Microorganisms From Sediments of a Hypersaline Pond in Atacama Desert, Chile.Frontiers in microbiology · 2021Article
- Pseudouridines of tRNA Anticodon Stem-Loop Have Unexpected Role in Mutagenesis inMicroorganisms · 2020Article
- Structural, biochemical and functional analyses of tRNA-monooxygenase enzyme MiaE from Pseudomonas putida provide insights into tRNA/MiaE interaction.Nucleic acids research · 2020Article
- A Structural Basis for Restricted Codon Recognition Mediated by 2-thiocytidine in tRNA Containing a Wobble Position Inosine.Journal of molecular biology · 2020Article
- Computational and NMR studies of RNA duplexes with an internal pseudouridine-adenosine base pair.Scientific reports · 2019Article
- Celebrating wobble decoding: Half a century and still much is new.RNA biology · 2018Review
- Transcriptome analysis reveals a stress response of Shewanella oneidensis deprived of background levels of ionizing radiation.PloS one · 2018Article
- mRNA pseudouridylation affects RNA metabolism in the parasiteRNA (New York, N.Y.) · 2017Article
- Structure modulation of helix 69 from Escherichia coli 23S ribosomal RNA by pseudouridylations.Nucleic acids research · 2014Article
- Pseudouridine: still mysterious, but never a fake (uridine)!RNA biology · 2014Review
- Article
- Solution nuclear magnetic resonance analyses of the anticodon arms of proteinogenic and nonproteinogenic tRNA(Gly).Biochemistry · 2012Article
- Conformation effects of base modification on the anticodon stem-loop of Bacillus subtilis tRNA(Tyr).Journal of molecular biology · 2011Article
- Effect of pseudouridylation on the structure and activity of the catalytically essential P6.1 hairpin in human telomerase RNA.Nucleic acids research · 2010Article
- The crystal structure of unmodified tRNAPhe from Escherichia coli.Nucleic acids research · 2010Article
- Nucleotide modifications in three functionally important regions of the Saccharomyces cerevisiae ribosome affect translation accuracy.Nucleic acids research · 2009Article
- RluD, a highly conserved pseudouridine synthase, modifies 50S subunits more specifically and efficiently than free 23S rRNA.RNA (New York, N.Y.) · 2007Article
- A counterintuitive Mg2+-dependent and modification-assisted functional folding of mitochondrial tRNAs.Journal of molecular biology · 2006Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Nucleoside base modifications can alter the structures and dynamics of RNA molecules and are important in tRNAs for maintaining translational fidelity and efficiency. The unmodified anticodon stem-loop from Escherichia coli tRNA(Phe) forms a trinucleotide loop in solution, but Mg2+ and dimethylallyl modification of A37 N6 destabilize the loop-proximal base pairs and increase the mobility of the loop nucleotides. The anticodon arm has three additional modifications, psi32, psi39, and A37 C2-thiomethyl. We have used NMR spectroscopy to investigate the structural and dynamical effects of psi32 on the anticodon stem-loop from E.coli tRNA(Phe). The psi32 modification does not significantly alter the structure of the anticodon stem-loop relative to the unmodified parent molecule. The stem of the RNA molecule includes base pairs psi32-A38 and U33-A37 and the base of psi32 stacks between U33 and A31. The glycosidic bond of psi32 is in the anti configuration and is paired with A38 in a Watson-Crick geometry, unlike residue 32 in most crystal structures of tRNA. The psi32 modification increases the melting temperature of the stem by approximately 3.5 degrees C, although the psi32 and U33 imino resonances are exchange broadened. The results suggest that psi32 functions to preserve the stem integrity in the presence of additional loop modifications or after reorganization of the loop into a translationally functional conformation.
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