ArticleAntimicrobial agents and chemotherapy2006
Simultaneous detection of nine antibiotic resistance-related genes in Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization assay.
Article in Antimicrobial agents and chemotherapy, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 64 citations in OpenAlex.
- A Longitudinal Study of Antimicrobial Resistance in Oral Bacterial Isolates From Periodontitis Patients in Taiwan.International dental journal · 2026Article
- Genomic characterization of group BMicrobial genomics · 2025Article
- Molecular characterization of Streptococcus agalactiae strains isolated from pregnant women.Scientific reports · 2025Article
- The antibacterial efficacy and effect of tungsten nanoparticles (WOGMS hygiene and infection control · 2025Article
- Development and performance evaluation of a qPCR-based assay for the fully automated detection of group BMicrobiology spectrum · 2024Article
- Antibiotic resistance genes and molecular typing of Streptococcus agalactiae isolated from pregnant women.BMC pregnancy and childbirth · 2023Article
- Epidemiology, Antimicrobial Resistance, and Virulence Determinants of Group BFrontiers in microbiology · 2022Article
- Article
- Prevalence and molecular characterization of group B streptococcus in pregnant women from hospitals in Ohangwena and Oshikoto regions of Namibia.BMC microbiology · 2021Article
- Virulence factors and antibiotic resistance properties of Streptococcus species isolated from hospital cockroaches.3 Biotech · 2021Article
- Antimicrobial profile of multidrug-resistantJournal of advanced veterinary and animal research · 2020Article
- Genomic characterisation of perinatal Western Australian Streptococcus agalactiae isolates.PloS one · 2019Article
- Perinatal Streptococcus agalactiae Epidemiology and Surveillance Targets.Clinical microbiology reviews · 2018Review
- Antibiotic resistance of Streptococcus agalactiae isolated from pregnant women in Garankuwa, South Africa.BMC research notes · 2015Article
- Molecular-based screening for perinatal group B streptococcal infection: implications for prevention and therapy.Molecular diagnosis & therapy · 2013Review
- Environmental impact assessment of Attenda abattoir, Ogbomoso southwestern Nigeria on surface and groundwater quality using geo-electrical imaging and microbiological analysis.Environmental monitoring and assessment · 2012Article
- Multiplex PCR-based reverse line blot assay for simultaneous detection of 22 virulence genes in uropathogenic Escherichia coli.Applied and environmental microbiology · 2012Article
- Multiplex PCR and reverse line blot hybridization assay (mPCR/RLB).Journal of visualized experiments : JoVE · 2011Article
- Diversity and mobility of integrative and conjugative elements in bovine isolates of Streptococcus agalactiae, S. dysgalactiae subsp. dysgalactiae, and S. uberis.Applied and environmental microbiology · 2010Article
- Antimicrobial resistance in colonizing group B Streptococci before the implementation of a Swedish intrapartum antibiotic prophylaxis program.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2010Article
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus agalactiae (group B streptococcus [GBS]) is the leading cause of neonatal and maternal sepsis. Penicillin is recommended for intrapartum prophylaxis, but erythromycin or clindamycin is used for penicillin-allergic carriers. Antibiotic resistance (AR) has increased recently and needs to be monitored. We have developed a multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay to detect, simultaneously, seven genes encoding AR--erm(A/TR), erm(B), mef(A/E), tet(M), tet(O), aphA-3, and aad-6--and two AR-related genes, int-Tn and mreA. We tested 512 GBS isolates from Asia and Australasia and compared mPCR/RLB with antibiotic susceptibility phenotype or single-gene PCR. Phenotypic resistance to tetracycline was identified in 450 (88%) isolates, of which 442 had tet(M) (93%) and/or tet(O) (6%). Of 67 (13%) erythromycin-resistant isolates, 18 were susceptible to clindamycin, i.e., had the M phenotype, encoded by mef(A/E); 39 had constitutive (cMLS(B)) and 10 inducible clindamycin resistance, and of these, 34 contained erm(B) and 12 erm(A/TR). Of four additional isolates with mef(A/E), three contained erm(B) with cMLS(B) and one was erythromycin susceptible. Of 61 (12%) clindamycin-resistant isolates, 20 were susceptible to erythromycin and two had intermediate resistance. Based on sequencing, 21 of 22 isolates with mef had mef(E), and 8 of 353 with int-Tn had an atypical sequence. Several AR genes, erm(B), tet(O), aphA-3, aad-6, and mef(A/E), were significantly more common among Asian than Australasian isolates, and there were significant differences in distribution of AR genes between GBS serotypes. Our mPCR/RLB assay is simple, rapid, and suitable for surveillance of antibiotic resistance in GBS.
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