ArticleRNA (New York, N.Y.)2006
A general two-step strategy to synthesize lariat RNAs.
Article in RNA (New York, N.Y.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Beyond the Sequence: Chemical and Topological Design and Innovations in mRNA Therapeutics.Chemical reviews · 2026Review
- Circular RNA vaccine in disease prevention and treatment.Signal transduction and targeted therapy · 2023Review
- RNA circularization strategies in vivo and in vitro.Nucleic acids research · 2015Review
- 2'-Azido RNA, a versatile tool for chemical biology: synthesis, X-ray structure, siRNA applications, click labeling.ACS chemical biology · 2012Article
- Deoxyribozymes: selection design and serendipity in the development of DNA catalysts.Accounts of chemical research · 2009Review
- Use of deoxyribozymes in RNA research.Methods in enzymology · 2009Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
We describe a general and efficient two-step strategy for lariat RNA synthesis. In the first step, a deoxyribozyme synthesizes 2',5'-branched RNA. In the second step, T4 RNA ligase closes the loop that completes the lariat. The loop-closure reaction can form either a natural or unnatural lariat isomer, depending on which of the two 3'-termini of the branched RNA reacts with the lone 5'-end. We demonstrate two approaches to control formation of either lariat isomer. In conjunction with other routes for lariat RNA synthesis, the two-step strategy described here will facilitate biochemical studies that require lariat RNAs of varying nucleotide sequence.
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Registered trials
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