ArticleMolecular biology of the cell2006
Shiga toxin regulates its entry in a Syk-dependent manner.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 75 citations in OpenAlex.
- Cellular signaling pathway of Shiga toxin-induced ATP release.Frontiers in cellular and infection microbiology · 2026Article
- Review
- Verotoxin Receptor-Based Pathology and Therapies.Frontiers in cellular and infection microbiology · 2020Review
- Protein Toxins That Utilize Gangliosides as Host Receptors.Progress in molecular biology and translational science · 2018Review
- Cholera toxin B subunit induces local curvature on lipid bilayers.FEBS open bio · 2017Article
- Protection against Shiga Toxins.Toxins · 2017Review
- Cross-linking of glycosphingolipids at the plasma membrane: consequences for intracellular signaling and traffic.Cellular and molecular life sciences : CMLS · 2016Article
- Activation of the Classical Mitogen-Activated Protein Kinases Is Part of the Shiga Toxin-Induced Ribotoxic Stress Response and May Contribute to Shiga Toxin-Induced Inflammation.Infection and immunity · 2016Article
- Microtubule motors power plasma membrane tubulation in clathrin-independent endocytosis.Traffic (Copenhagen, Denmark) · 2015Article
- Building endocytic pits without clathrin.Nature reviews. Molecular cell biology · 2015Review
- The adaptor protein GULP promotes Jedi-1-mediated phagocytosis through a clathrin-dependent mechanism.Molecular biology of the cell · 2014Article
- Retrograde trafficking of AB₅ toxins: mechanisms to therapeutics.Journal of molecular medicine (Berlin, Germany) · 2013Review
- Identification of a peptide-based neutralizer that potently inhibits both Shiga toxins 1 and 2 by targeting specific receptor-binding regions.Infection and immunity · 2013Article
- The B subunits of Shiga-like toxins induce regulated VWF secretion in a phospholipase D1-dependent manner.Blood · 2012Article
- Ricin and Shiga toxins: effects on host cell signal transduction.Current topics in microbiology and immunology · 2012Review
- Article
- Article
- Lipid-mediated endocytosis.Cold Spring Harbor perspectives in biology · 2011Review
- Shiga toxin and its use in targeted cancer therapy and imaging.Microbial biotechnology · 2011Review
- Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shiga toxin (Stx) is composed of an A-moiety that inhibits protein synthesis after translocation into the cytosol, and a B-moiety that binds to Gb3 at the cell surface and mediates endocytosis of the toxin. After endocytosis, Stx is transported retrogradely to the endoplasmic reticulum, and then the A-fragment enters the cytosol. In this study, we have investigated whether toxin-induced signaling is involved in its entry. Stx was found to activate Syk and induce rapid tyrosine phosphorylation of several proteins, one protein being clathrin heavy chain. Toxin-induced clathrin phosphorylation required Syk activity, and in cells overexpressing Syk, a complex containing clathrin and Syk could be demonstrated. Depletion of Syk by small interfering RNA, expression of a dominant negative Syk mutant (Syk KD), or treatment with the Syk inhibitor piceatannol inhibited not only Stx-induced clathrin phosphorylation but also endocytosis of the toxin. Also, Golgi transport of Stx was inhibited under all these conditions. In conclusion, our data suggest that Stx regulates its entry into target cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.