ArticleMolecular biology of the cell2006
Rapid, diffusional shuttling of poly(A) RNA between nuclear speckles and the nucleoplasm.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 93 citations in OpenAlex.
- Phosphorylation of a nuclear condensate regulates cohesion and mRNA retention.Nature communications · 2025Article
- Splicing regulation through biomolecular condensates and membraneless organelles.Nature reviews. Molecular cell biology · 2024Review
- Ethanol- and PARP-Mediated Regulation of Ribosome-Associated Long Non-Coding RNA (lncRNA) in Pyramidal Neurons.Non-coding RNA · 2023Article
- An Intermittent Cytochemist.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
- It's Just a Phase: Exploring the Relationship Between mRNA, Biomolecular Condensates, and Translational Control.Frontiers in genetics · 2022Review
- Nuclear mechanisms of gene expression control: pre-mRNA splicing as a life or death decision.Current opinion in genetics & development · 2021Review
- Splicing at the phase-separated nuclear speckle interface: a model.Nucleic acids research · 2021Review
- Article
- Dynamical reorganization of the pluripotency transcription factors Oct4 and Sox2 during early differentiation of embryonic stem cells.Scientific reports · 2020Article
- Uncoupling of nucleo-cytoplasmic RNA export and localization during stress.Nucleic acids research · 2019Article
- Nuclear speckles: molecular organization, biological function and role in disease.Nucleic acids research · 2017Review
- Giant poly(A)-rich RNP aggregates form at terminal regions of avian lampbrush chromosomes.Chromosoma · 2016Article
- Probability-based particle detection that enables threshold-free and robust in vivo single-molecule tracking.Molecular biology of the cell · 2015Article
- ECHO-liveFISH: in vivo RNA labeling reveals dynamic regulation of nuclear RNA foci in living tissues.Nucleic acids research · 2015Article
- Nuclear accessibility of β-actin mRNA is measured by 3D single-molecule real-time tracking.The Journal of cell biology · 2015Article
- The conserved ubiquitin-like protein Hub1 plays a critical role in splicing in human cells.Journal of molecular cell biology · 2014Article
- To the pore and through the pore: a story of mRNA export kinetics.Biochimica et biophysica acta · 2012Review
- Photoactivatable fluorophores and techniques for biological imaging applications.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2012Review
- Post-transcriptional spliceosomes are retained in nuclear speckles until splicing completion.Nature communications · 2012Article
- The nucleus introduced.Cold Spring Harbor perspectives in biology · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Speckles are nuclear bodies that contain pre-mRNA splicing factors and polyadenylated RNA. Because nuclear poly(A) RNA consists of both mRNA transcripts and nucleus-restricted RNAs, we tested whether poly(A) RNA in speckles is dynamic or rather an immobile, perhaps structural, component. Fluorescein-labeled oligo(dT) was introduced into HeLa cells stably expressing a red fluorescent protein chimera of the splicing factor SC35 and allowed to hybridize. Fluorescence correlation spectroscopy (FCS) showed that the mobility of the tagged poly(A) RNA was virtually identical in both speckles and at random nucleoplasmic sites. This same result was observed in photoactivation-tracking studies in which caged fluorescein-labeled oligo(dT) was used as hybridization probe, and the rate of movement away from either a speckle or nucleoplasmic site was monitored using digital imaging microscopy after photoactivation. Furthermore, the tagged poly(A) RNA was observed to rapidly distribute throughout the entire nucleoplasm and other speckles, regardless of whether the tracking observations were initiated in a speckle or the nucleoplasm. Finally, in both FCS and photoactivation-tracking studies, a temperature reduction from 37 to 22 degrees C had no discernible effect on the behavior of poly(A) RNA in either speckles or the nucleoplasm, strongly suggesting that its movement in and out of speckles does not require metabolic energy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.