ArticlePlant cell reports2006
The maize Knotted1 gene is an effective positive selectable marker gene for Agrobacterium-mediated tobacco transformation.
Article in Plant cell reports, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Advances in the use of morphogenic regulators and peptide regenerating factors for boosting plant transformation and genome editing.Frontiers in plant science · 2026Review
- Improving transformation and regeneration efficiency in medicinal plants: insights from other recalcitrant species.Journal of experimental botany · 2025Review
- Appreciating animal induced pluripotent stem cells to shape plant cell reprogramming strategies.Journal of experimental botany · 2024Review
- Promoting genotype-independent plant transformation by manipulating developmental regulatory genes and/or using nanoparticles.Plant cell reports · 2023Review
- Genotype-independent plant transformation.Horticulture research · 2022Article
- Advances and Perspectives in Tissue Culture and Genetic Engineering of Cannabis.International journal of molecular sciences · 2021Review
- Using Morphogenic Genes to Improve Recovery and Regeneration of Transgenic Plants.Plants (Basel, Switzerland) · 2019Review
- The antioxidative defense system is involved in the premature senescence in transgenic tobacco (Nicotiana tabacum NC89).Biological research · 2016Article
- Promoter analysis of the sweet potato ADP-glucose pyrophosphorylase gene IbAGP1 in Nicotiana tabacum.Plant cell reports · 2015Article
- Split-Cre complementation restores combination activity on transgene excision in hair roots of transgenic tobacco.PloS one · 2014Article
- Isolation and characterization of cDNAs encoding leucoanthocyanidin reductase and anthocyanidin reductase from Populus trichocarpa.PloS one · 2013Article
- Molecular cloning and characterization of PtrLAR3, a gene encoding leucoanthocyanidin reductase from Populus trichocarpa, and its constitutive expression enhances fungal resistance in transgenic plants.Journal of experimental botany · 2012Article
- Recent advances in development of marker-free transgenic plants: regulation and biosafety concern.Journal of biosciences · 2012Review
- Molecular cloning and characterization of two genes encoding dihydroflavonol-4-reductase from Populus trichocarpa.PloS one · 2012Article
- Characterization of SSU5C promoter of a rbcS gene from duckweed (Lemna gibba).Molecular biology reports · 2011Article
- Lysine racemase: a novel non-antibiotic selectable marker for plant transformation.Plant molecular biology · 2010Article
- Functional analysis of the Arabidopsis thaliana poly(A) binding protein PAB5 gene promoter in Nicotiana tabacum.Plant cell reports · 2008Article
- Functional characterization of a cotton late embryogenesis-abundant D113 gene promoter in transgenic tobacco.Plant cell reports · 2008Article
- Leaf senescence is delayed in tobacco plants expressing the maize knotted1 gene under the control of a wound-inducible promoter.Plant cell reports · 2006Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have assessed the use of a homeobox gene knotted1 (kn1) from maize as a selectable marker gene for plant transformation. The kn1 gene under the control of cauliflower mosaic virus 35S promoter (35S::kn1) was introduced into Nicotiana tabacum cv. Xanthi via Agrobacterium-mediated transformation. Under nonselective conditions (without antibiotic selection) on a hormone-free medium (MS), a large number of transgenic calli and shoots were obtained from explants that were infected with Agrobacterium tumefaciens LBA4404 harboring the 35S::kn1 gene. On the other hand, no calli or shoots were produced from explants that were infected with an Agrobacterium strain harboring pBI121 (nptII selection) or from uninfected controls cultured under identical conditions. Relative to kanamycin selection conferred by nptII, the use of kn1 resulted in a 3-fold increase in transformation efficiency. The transgenic status of shoots obtained was confirmed by both histochemical detection of GUS activity and molecular analysis. The results presented here suggest that kn1 gene could be used as an effective alternative selection marker with a potential to enhance plant transformation efficiency in many plant species. With kn1 gene as a selection marker gene, no antibiotic-resistance or herbicide-resistance genes are needed so that potential risks associated with the use of these traditional selection marker genes can be eliminated.
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