ArticleInfection and immunity2006
Microbial pattern recognition receptors mediate M-cell uptake of a gram-negative bacterium.
Article in Infection and immunity, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 96 citations in OpenAlex.
- A prospective, randomized, blinded, placebo-controlled pilot study on the effect of Enterococcus faecium on clinical activity and intestinal gene expression in canine food-responsive chronic enteropathy.Journal of veterinary internal medicineTrial
- The intestinal interferon system and specialized enterocytes as putative drivers of HIV latency.Frontiers in immunology · 2025Review
- Intestinal Peyer's Patches: Structure, Function, and In Vitro Modeling.Tissue engineering and regenerative medicine · 2023Review
- Oral Biologic Delivery: Advances Toward Oral Subunit, DNA, and mRNA Vaccines and the Potential for Mass Vaccination During Pandemics.Annual review of pharmacology and toxicology · 2021Review
- Hyaluronic Acid in the Intestinal Tract: Influence of Structure, Rheology, and Mucoadhesion on the Intestinal Uptake in Rats.Biomolecules · 2020Article
- Article
- Thermoresponsive Bacteriophage Nanocarrier as a Gene Delivery Vector Targeted to the Gastrointestinal Tract.Molecular therapy. Nucleic acids · 2018Article
- Differential expression of Toll-like receptor-2, -4 and -9 in follicle-associated epithelium from epithelia of both follicle-associated intestinal villi and ordinary intestinal villi in rat Peyer's patches.The Journal of veterinary medical science · 2017Article
- The development of mucosal vaccines for both mucosal and systemic immune induction and the roles played by adjuvants.Clinical and experimental vaccine research · 2017Review
- Antigen targeting to M cells for enhancing the efficacy of mucosal vaccines.Experimental & molecular medicine · 2014Review
- Blowing on embers: commensal microbiota and our immune system.Frontiers in immunology · 2014Review
- Enhancement of serum and mucosal immune responses to a Haemophilus influenzae Type B vaccine by intranasal delivery.Clinical and vaccine immunology : CVI · 2013Article
- Dectin-1 is essential for reverse transcytosis of glycosylated SIgA-antigen complexes by intestinal M cells.PLoS biology · 2013Article
- The SRC family tyrosine kinase HCK and the ETS family transcription factors SPIB and EHF regulate transcytosis across a human follicle-associated epithelium model.The Journal of biological chemistry · 2013Article
- Mucosal Immune System and M Cell-targeting Strategies for Oral Mucosal Vaccination.Immune network · 2012Article
- Examining the role of nasopharyngeal-associated lymphoreticular tissue (NALT) in mouse responses to vaccines.Journal of visualized experiments : JoVE · 2012Article
- The role of innate immunity in the host defense against intestinal bacterial pathogens.Current infectious disease reports · 2012Article
- Nasal immunity to staphylococcal toxic shock is controlled by the nasopharynx-associated lymphoid tissue.Clinical and vaccine immunology : CVI · 2011Article
- Salmonella Interaction with and Passage through the Intestinal Mucosa: Through the Lens of the Organism.Frontiers in microbiology · 2011Article
- Epithelial cell coculture models for studying infectious diseases: benefits and limitations.Journal of biomedicine & biotechnology · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The receptors involved in the sampling of particulate microbial antigens by the gut are largely unknown. Here we demonstrate for the first time in an in vitro M-cell model and in situ in isolated murine intestinal segments that the receptors TLR-4, PAF-R, and alpha5beta1 integrin are all involved in mediating bacterial uptake associated with transcytosis. The pattern of expression of TLR-4 and alpha5beta1 integrin differed between M cells and enterocytes. There was increased apical expression of TLR-4 in M-cell cultures, and it was present on the apical surface of murine M cells but not enterocytes in situ. In contrast, PAF-R was expressed equally by both cell types in vitro and was abundantly expressed throughout the intestinal epithelium. Inhibition of TLR-4 and PAF-R, but not TLR-2, reduced gram-negative bacterial uptake by both cell types, whereas inhibition of the apically expressed alpha5beta1 integrin significantly reduced the ability of M cells to translocate bacteria. Hence, the involvement of each receptor was dependent not only on differences in the level of receptor expression but the cellular localization. Using bacteria that had mutations that affected the bacterial lipooligosaccharide structure indicated that the oligosaccharide moiety was important in bacterial uptake. Taken together, the data suggest that pathogen-associated molecular pattern interactions with pattern recognition receptors are key factors in M-cell recognition of intestinal antigens for mucosal immune priming.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.