ArticleInfection and immunity2006
Transcriptional activation of the Staphylococcus aureus putP gene by low-proline-high osmotic conditions and during infection of murine and human tissues.
Article in Infection and immunity, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
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- Proline transporters ProT and PutP are required for Staphylococcus aureus infection.PLoS pathogens · 2023Article
- Proline Transport and Growth Changes in Proline Transport Mutants ofMicroorganisms · 2022Article
- Prokaryotic Solute/Sodium Symporters: Versatile Functions and Mechanisms of a Transporter Family.International journal of molecular sciences · 2021Review
- Uropathogens Preferrentially Interact with Conditioning Film Components on the Surface of Indwelling Ureteral Stents Rather than Stent Material.Pathogens (Basel, Switzerland) · 2020Article
- High-throughput transposon sequencing highlights the cell wall as an important barrier for osmotic stress in methicillin resistant Staphylococcus aureus and underlines a tailored response to different osmotic stressors.Molecular microbiology · 2020Article
- Role of Proline in Pathogen and Host Interactions.Antioxidants & redox signaling · 2019Review
- Structural and metabolic responses of Staphylococcus aureus biofilms to hyperosmotic and antibiotic stress.Biotechnology and bioengineering · 2018Article
- Temporal Regulation ofJournal of pathogens · 2017Article
- The second messenger c-di-AMP inhibits the osmolyte uptake system OpuC in Staphylococcus aureus.Science signaling · 2016Article
- Selection of suitable reference genes for gene expression studies in Staphylococcus capitis during growth under erythromycin stress.Molecular genetics and genomics : MGG · 2016Article
- Glu-311 in External Loop 4 of the Sodium/Proline Transporter PutP Is Crucial for External Gate Closure.The Journal of biological chemistry · 2016Article
- Mutational and transcriptional analyses of the Staphylococcus aureus low-affinity proline transporter OpuD during in vitro growth and infection of murine tissues.FEMS immunology and medical microbiology · 2011Article
- Tool for quantification of staphylococcal enterotoxin gene expression in cheese.Applied and environmental microbiology · 2010Article
- Backbone structure of transmembrane domain IX of the Na+/proline transporter PutP of Escherichia coli.Biophysical journal · 2009Article
- Osmolyte transport inWorld journal of clinical infectious diseasesArticle
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Staphylococcus aureus can grow virtually anywhere in the human body but needs to import proline through low- and high-affinity proline transporters to survive. This study examined the regulation of the S. aureus putP gene, which encodes a high-affinity proline permease. putP::lacZ and putP::lux transcriptional fusions were constructed and integrated into the genomes of several S. aureus strains. Enzyme activity was measured after growth in media with various osmolyte concentrations. As osmolarity rose, putP expression increased, with a plateau at 2 M for NaCl in strain LL3-1. Proline concentrations as low as 17.4 muM activated expression of the putP gene. The putP::lux fusion was also integrated into the genomes of S. aureus strains that were either SigB inactive (LL3-1, 8325-4, and SH1003) or SigB active (Newman and SH1000). SigB inactive strains showed increased putP gene expression as NaCl concentrations rose, whereas SigB active strains displayed a dramatic decrease in putP expression, suggesting that the alternative sigma factor B plays a negative role in putP regulation. Mice inoculated with S. aureus strains containing the putP::lux fusion exhibited up to a 715-fold increase in putP expression, although levels in the various murine organs differed. Moreover, urine from human patients infected with S. aureus showed elevated putP levels by use of a PCR procedure, whereas blood and some abscess material had no significant increase. Thus, putP is transcriptionally activated by a low-proline and high osmotic environment both in growth media and in murine or human clinical specimens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.