ArticleInfection and immunity2006
Enterocyte cytoskeleton changes are crucial for enhanced translocation of nonpathogenic Escherichia coli across metabolically stressed gut epithelia.
Article in Infection and immunity, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 58 citations in OpenAlex.
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- Crohn's Disease Pathobiont Adherent-Invasive E coli Disrupts Epithelial Mitochondrial Networks With Implications for Gut Permeability.Cellular and molecular gastroenterology and hepatology · 2021Article
- Clinical relevance of intestinal barrier dysfunction in common gastrointestinal diseases.World journal of gastrointestinal pathophysiology · 2020Review
- Introducing variability in targeting the microtubules: Review of current mechanisms and future directions in colchicine therapy.Pharmacology research & perspectives · 2020Review
- Biogenic selenium nanoparticles synthesized byInternational journal of nanomedicine · 2019Article
- Biosynthesis of Polysaccharides-Capped Selenium Nanoparticles UsingFrontiers in microbiology · 2019Article
- Reduced intestinal epithelial mitochondrial function enhances in vitro interleukin-8 production in response to commensal Escherichia coli.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2018Article
- Helminths and intestinal barrier function.Tissue barriers · 2017Review
- Claudin-3 and occludin tissue content in the glands of colonic mucosa with and without a fecal stream.Journal of molecular histology · 2015Article
- Targeting mitochondria-derived reactive oxygen species to reduce epithelial barrier dysfunction and colitis.The American journal of pathology · 2014Article
- Differential effect of oxidative stress on intestinal apparent permeability of drugs transported by paracellular and transcellular route.European journal of drug metabolism and pharmacokinetics · 2012Article
- Indomethacin-induced translocation of bacteria across enteric epithelia is reactive oxygen species-dependent and reduced by vitamin C.American journal of physiology. Gastrointestinal and liver physiology · 2012Article
- Occludin regulates macromolecule flux across the intestinal epithelial tight junction barrier.American journal of physiology. Gastrointestinal and liver physiology · 2011Article
- In vivo actin cross-linking induced by Vibrio cholerae type VI secretion system is associated with intestinal inflammation.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Elevated systemic antibodies towards commensal gut microbiota in autoinflammatory condition.PloS one · 2008Article
- The role of clathrin-dependent endocytosis in bacterial internalization.Trends in cell biology · 2006Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Substantial data implicate the commensal flora as triggers for the initiation of enteric inflammation or inflammatory disease relapse. We have shown that enteric epithelia under metabolic stress respond to nonpathogenic bacteria by increases in epithelial paracellular permeability and bacterial translocation. Here we assessed the structural basis of these findings. Confluent filter-grown monolayers of the human colonic T84 epithelial cell line were treated with 0.1 mM dinitrophenol (which uncouples oxidative phosphorylation) and noninvasive, nonpathogenic Escherichia coli (strain HB101, 10(6) CFU) with or without pretreatment with various pharmacological agents. At 24 h later, apoptosis, tight-junction protein expression, transepithelial resistance (TER; a marker of paracellular permeability), and bacterial internalization and translocation were assessed. Treatment with stabilizers of microtubules (i.e., colchicine), microfilaments (i.e., jasplakinolide) and clathrin-coated pit endocytosis (i.e., phenylarsine oxide) all failed to block DNP+E. coli HB101-induced reductions in TER but effectively prevented bacterial internalization and translocation. Neither the TER defect nor the enhanced bacterial translocations were a consequence of increased apoptosis. These data show that epithelial paracellular and transcellular (i.e., bacterial internalization) permeation pathways are controlled by different mechanisms. Thus, epithelia under metabolic stress increase their endocytotic activity that can result in a microtubule-, microfilament-dependent internalization and transcytosis of bacteria. We speculate that similar events in vivo would allow excess unprocessed antigen and bacteria into the mucosa and could evoke an inflammatory response by, for example, the activation of resident or recruited immune cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.