ArticleThe Journal of cell biology2005
Alpha4beta1-dependent adhesion strengthening under mechanical strain is regulated by paxillin association with the alpha4-cytoplasmic domain.
Article in The Journal of cell biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 92 citations in OpenAlex.
- PI3Kγ in Tumour Inflammation: Bridging Immune Response and Cancer Progression-A Mini-Review.Immunology · 2025Review
- CD32B1, a versatile non-signaling antibody-binding scaffold for enhanced T cell adhesion to tumor stromal cognate antigens.Frontiers in immunology · 2025Article
- Review
- PI3Kγ stimulates a high molecular weight form of myosin light chain kinase to promote myeloid cell adhesion and tumor inflammation.Nature communications · 2022Article
- CD44 engagement enhances acute myeloid leukemia cell adhesion to the bone marrow microenvironment by increasing VLA-4 avidity.Haematologica · 2021Article
- Leukaemia: a model metastatic disease.Nature reviews. Cancer · 2021Review
- Prion Protein at the Leading Edge: Its Role in Cell Motility.International journal of molecular sciences · 2020Review
- Go with the flow: GEF-H1 mediated shear stress mechanotransduction in neutrophils.Small GTPases · 2020Review
- Mechanical Communication at the Immunological Synapse.Trends in cell biology · 2017Review
- PARP inhibition in leukocytes diminishes inflammation via effects on integrins/cytoskeleton and protects the blood-brain barrier.Journal of neuroinflammation · 2016Article
- The Lhx9-integrin pathway is essential for positioning of the proepicardial organ.Development (Cambridge, England) · 2016Article
- Heparanase-induced shedding of syndecan-1/CD138 in myeloma and endothelial cells activates VEGFR2 and an invasive phenotype: prevention by novel synstatins.Oncogenesis · 2016Article
- Increasing throughput of AFM-based single cell adhesion measurements through multisubstrate surfaces.Beilstein journal of nanotechnology · 2015Article
- Specific phosphorylations transmit signals from leukocyte β2 to β1 integrins and regulate adhesion.The Journal of biological chemistry · 2014Article
- Mechanosensitive kinases regulate stiffness-induced cardiomyocyte maturation.Scientific reports · 2014Article
- B-Raf regulation of integrin α4β1-mediated resistance to shear stress through changes in cell spreading and cytoskeletal association in T cells.The Journal of biological chemistry · 2014Article
- Integrin αII b tail distal of GFFKR participates in inside-out αII b β3 activation.Journal of thrombosis and haemostasis : JTH · 2014Article
- Article
- α Integrin cytoplasmic tails have tissue-specific roles during C. elegans development.The International journal of developmental biology · 2014Article
- Elongated membrane tethers, individually anchored by high affinity α4β1/VCAM-1 complexes, are the quantal units of monocyte arrests.PloS one · 2013Article
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 3 countries.
Funding
Abstract
The capacity of integrins to mediate adhesiveness is modulated by their cytoplasmic associations. In this study, we describe a novel mechanism by which alpha4-integrin adhesiveness is regulated by the cytoskeletal adaptor paxillin. A mutation of the alpha4 tail that disrupts paxillin binding, alpha4(Y991A), reduced talin association to the alpha4beta1 heterodimer, impaired integrin anchorage to the cytoskeleton, and suppressed alpha4beta1-dependent capture and adhesion strengthening of Jurkat T cells to VCAM-1 under shear stress. The mutant retained intrinsic avidity to soluble or bead-immobilized VCAM-1, supported normal cell spreading at short-lived contacts, had normal alpha4-microvillar distribution, and responded to inside-out signals. This is the first demonstration that cytoskeletal anchorage of an integrin enhances the mechanical stability of its adhesive bonds under strain and, thereby, promotes its ability to mediate leukocyte adhesion under physiological shear stress conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.