ArticleJournal of cellular and molecular medicine
Impact of electrical stimulation on three-dimensional myoblast cultures - a real-time RT-PCR study.
Article in Journal of cellular and molecular medicine. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Exploring the piezoelectric phenomenon: From polymers to human tissues and advanced applications in tissue engineering.Bioactive materials · 2026Review
- Electrically Conductive Materials: Opportunities and Challenges in Tissue Engineering.Biomolecules · 2019Review
- Extracellular matrix remodelling induced by alternating electrical and mechanical stimulations increases the contraction of engineered skeletal muscle tissues.Scientific reports · 2019Article
- Biomaterials in Tendon and Skeletal Muscle Tissue Engineering: Current Trends and Challenges.Materials (Basel, Switzerland) · 2018Review
- Effect of PEDOT:PSS in tissue engineering composite scaffold on improvement and maintenance of endothelial cell function.Journal of biosciences · 2018Article
- The Importance of Biophysical and Biochemical Stimuli in Dynamic Skeletal Muscle Models.Frontiers in physiology · 2018Review
- Characterization and optimization of actuating poly(ethylene glycol) diacrylate/acrylic acid hydrogels as artificial muscles.Polymer · 2017Article
- Neuromuscular electrical stimulation promotes development in mice of mature human muscle from immortalized human myoblasts.Skeletal muscle · 2016Article
- Tissue engineered strategies for skeletal muscle injury.Stem cells international · 2012Review
- Electric pulse stimulation of cultured murine muscle cells reproduces gene expression changes of trained mouse muscle.PloS one · 2010Article
- Collagen matrices from sponge to nano: new perspectives for tissue engineering of skeletal muscle.BMC biotechnology · 2009Article
- Effects of electrical stimulation in C2C12 muscle constructs.Journal of tissue engineering and regenerative medicine · 2008Article
- [Economic aspects of surgical wound therapies].Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen · 2008Article
- Review
- Tissue engineering in head and neck reconstructive surgery: what type of tissue do we need?European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2007Review
- Tissue engineered nerve constructs: where do we stand?Journal of cellular and molecular medicineReview
- Review
- A new approach to tissue engineering of vascularized skeletal muscle.Journal of cellular and molecular medicineArticle
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several focal skeletal muscle diseases, including tumours and trauma lead to a limited loss of functional muscle tissue. There is still no suitable clinical approach for treating such defects. A promising approach could be the tissue engineering of skeletal muscle. However, a clinically reliable differentiation stimulus for three-dimensional (3-D) cultures is necessary for this process, and this condition has not yet been established. In order to quantify and analyze the differentiation potential of electrical cell stimulation, primary myoblasts were stimulated within a 3-D fibrin- matrix. Gene expression of MyoD, myogenin and AChR-epsilon were measured by real-time RT-PCR over a time period of eight days, showing immediate down-regulation of all marker genes. For tissue engineering approaches, cell multiplication is crucial for acquisition of sufficient tissue volumes for reconstruction. Therefore, all experiments were performed with high and low passaged myoblasts, demonstrating higher transcript rates of marker genes in lowpassage cells. Our findings strongly suggest a reconsideration of electrical stimulation in muscle tissue engineering.
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