ArticleThe Biochemical journal2006
Enhanced levels of Pis1p (phosphatidylinositol synthase) improve the growth of Saccharomyces cerevisiae cells deficient in Rsp5 ubiquitin ligase.
Article in The Biochemical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 26 citations in OpenAlex.
- Lipid saturation controls nuclear envelope function.Nature cell biology · 2023Article
- How is the acyl chain composition of phosphoinositides created and does it matter?Biochemical Society transactions · 2019Review
- Determinants of the cytosolic turnover of mitochondrial intermembrane space proteins.BMC biology · 2018Article
- Ubiquitin-Activated Interaction Traps (UBAITs) identify E3 ligase binding partners.EMBO reports · 2015Article
- Saccharomyces cerevisiae essential genes with an Opi- phenotype.G3 (Bethesda, Md.) · 2014Article
- The yeast acyltransferase Sct1p regulates fatty acid desaturation by competing with the desaturase Ole1p.Molecular biology of the cell · 2012Article
- Rsp5 ubiquitin ligase is required for protein trafficking in Saccharomyces cerevisiae COPI mutants.PloS one · 2012Article
- The ubiquitin ligase Rsp5 is required for ribosome stability in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2011Article
- A lipid-mediated quality control process in the Golgi apparatus in yeast.Molecular biology of the cell · 2008Article
- Functional dissection of a HECT ubiquitin E3 ligase.Molecular & cellular proteomics : MCP · 2008Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Rsp5 ubiquitin ligase plays a role in many cellular processes including the biosynthesis of unsaturated fatty acids. The PIS1 (phosphatidylinositol synthase gene) encoding the enzyme Pis1p which catalyses the synthesis of phosphatidylinositol from CDP-diacyglycerol and inositol, was isolated in a screen for multicopy suppressors of the rsp5 temperature sensitivity phenotype. Suppression was allele non-specific. Interestingly, expression of PIS1 was 2-fold higher in the rsp5 mutant than in wild-type yeast, whereas the introduction of PIS1 in a multicopy plasmid increased the level of Pis1p 6-fold in both backgrounds. We demonstrate concomitantly that the expression of INO1 (inositol phosphate synthase gene) was also elevated approx. 2-fold in the rsp5 mutant as compared with the wild-type, and that inositol added to the medium improved growth of rsp5 mutants at a restrictive temperature. These results suggest that enhanced phosphatidylinositol synthesis may account for PIS1 suppression of rsp5 defects. Analysis of lipid extracts revealed the accumulation of saturated fatty acids in the rsp5 mutant, as a consequence of the prevention of unsaturated fatty acid synthesis. Overexpression of PIS1 did not correct the cellular fatty acid content; however, saturated fatty acids (C(16:0)) accumulated preferentially in phosphatidylinositol, and (wild-type)-like fatty acid composition in phosphatidylethanolamine was restored.
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