ArticleBiochemistry2005
Hydration changes in the association of Hoechst 33258 with DNA.
Article in Biochemistry, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 53 citations in OpenAlex.
- Epigallocatechin Gallate Affects the Structure of Chromatosomes, Nucleosomes and Their Complexes with PARP1.International journal of molecular sciences · 2023Article
- Optical Polymorphism of Liquid-Crystalline Dispersions of DNA at High Concentrations of Crowding Polymer.International journal of molecular sciences · 2023Article
- DNA minor-groove binder Hoechst 33258 destabilizes base-pairing adjacent to its binding site.Communications biology · 2020Article
- Investigation of the electrostatic and hydration properties of DNA minor groove-binding by a heterocyclic diamidine by osmotic pressure.Biophysical chemistry · 2017Article
- Addition of bases to the 5'-end of human telomeric DNA: influences on thermal stability and energetics of unfolding.Molecules (Basel, Switzerland) · 2014Article
- Ag(+)-mediated assembly of 5'-guanosine monophosphate.The journal of physical chemistry. B · 2010Article
- Minor groove binding compounds that jump a gc base pair and bind to adjacent AT base pair sites.Biochemistry · 2009Article
- Dissociation of minor groove binders from DNA: insights from metadynamics simulations.Nucleic acids research · 2008Article
- Break in the heat capacity change at 303 K for complex binding of netropsin to AATT containing hairpin DNA constructs.Biophysical journal · 2007Article
- Hydration changes accompanying the binding of minor groove ligands with DNA.Biophysical journal · 2007Article
- Protein phase diagrams II: nonideal behavior of biochemical reactions in the presence of osmolytes.Biophysical journal · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The role of water in the interaction of Hoechst 33258 with the minor groove binding site of the (AATT)2 sequence was investigated using calorimetric and equilibrium constant measurements. Using isothermal titration calorimetry measurements, the heat capacity change for the reaction is -256 +/- 10 cal/(K mol of Hoechst). Comparison with the heat capacity changes based on area models supports the expulsion of water from the interface of the Hoechst-DNA complex. To further consider the role of water, the osmotic stress method was used to determine if the Hoechst association with DNA was coupled with hydration changes. Using four osmolytes with varying molecular weights and chemical properties, the Hoechst affinity for DNA decreases with increasing osmolyte concentration. From the dependence of the equilibrium constant on the solution osmolality, 60 +/- 13 waters are acquired in the complex relative to the reactants. It is proposed that the osmotic stress technique is measuring weakly bound waters that are not measured via the heat capacity changes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.