ArticlePlanta2006
Changes in chloroplast DNA during development in tobacco, Medicago truncatula, pea, and maize.
Article in Planta, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
54 citing papers in PubMed, 111 citations in OpenAlex.
- Identification of toxic plants from poisonous samples using massively parallel sequencing.Forensic toxicology · 2026Article
- Biparental plastid inheritance and its implications in plastid genome engineering.Plant cell reports · 2025Review
- Cytoplasmic inheritance: The transmission of plastid and mitochondrial genomes across cells and generations.Plant physiology · 2025Review
- Production of functional human galectin-1 in transplastomic tobacco and simplified recovery via batch-mode purification.Frontiers in plant science · 2025Article
- A novel in-situ-process technique constructs whole circular cpDNA library.Plant methods · 2024Article
- An analysis of codon utilization patterns in the chloroplast genomes of three species of Coffea.BMC genomic data · 2023Article
- Oxidative and Glycation Damage to Mitochondrial DNA and Plastid DNA during Plant Development.Antioxidants (Basel, Switzerland) · 2023Review
- The Potential Role of Plastome Copy Number as a Quality Biomarker for Plant Products using Real-time Quantitative Polymerase Chain Reaction.Current genomics · 2022Article
- Glycation damage to organelles and their DNA increases during maize seedling development.Scientific reports · 2022Article
- Insights into the relevance between bacterial endophytic communities and resistance of rice cultivars infected by3 Biotech · 2021Article
- Detecting de novo mitochondrial mutations in angiosperms with highly divergent evolutionary rates.Genetics · 2021Article
- Advancing organelle genome transformation and editing for crop improvement.Plant communications · 2021Review
- Mitigation of deleterious phenotypes in chloroplast-engineered plants accumulating high levels of foreign proteins.Biotechnology for biofuels · 2021Article
- An Insight Into the Mechanism of Plant Organelle Genome Maintenance and Implications of Organelle Genome in Crop Improvement: An Update.Frontiers in cell and developmental biology · 2021Review
- Plastid Genomes of Flowering Plants: Essential Principles.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Transformation of the Plastid Genome in Tobacco: The Model System for Chloroplast Genome Engineering.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Unprecedented Intraindividual Structural Heteroplasmy in Eleocharis (Cyperaceae, Poales) Plastomes.Genome biology and evolution · 2020Article
- Reactive Oxygen Species, Antioxidant Agents, and DNA Damage in Developing Maize Mitochondria and Plastids.Frontiers in plant science · 2020Article
- A New Chloroplast DNA Extraction Protocol Significantly Improves the Chloroplast Genome Sequence Quality of Foxtail Millet (Setaria italica (L.) P. Beauv.).Scientific reports · 2019Article
- Plant Organelle Genome Replication.Plants (Basel, Switzerland) · 2019Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We examined the DNA from chloroplasts obtained from young and fully expanded leaves of tobacco (Nicotiana tabacum L.), Medicago truncatula, pea (Pisum sativum L.), and maize (Zea mays L.). The changes in plastid DNA content and structure were monitored by four independent methods: 4',6-diamidino-2-phenylindole (DAPI) staining with intact chloroplasts, in situ DAPI staining of cytological sections, ethidium bromide staining at the single-molecule level after exhaustive deproteinization of lysed chloroplasts, and pulsed-field gel electrophoresis. During leaf development, we found a decline of chloroplast DNA (cpDNA) in all four plants. For tobacco, for which plants can readily be regenerated from somatic cells, cpDNA persisted longer than in the other three plants. We also found a striking progression from complex multigenomic DNA molecules to simple subgenomic molecules during plastid development. Although the decrease in molecular size and complexity paralleled the decrease in DNA content per plastid, 6% of the chloroplasts in a fully expanded tobacco leaf still contained DNA in complex branched structure, whereas no such complex structures were found in mature leaves for the hard-to-regenerate maize.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.