ArticleBiophysical journal2006
Subcellular in vivo 1H MR spectroscopy of Xenopus laevis oocytes.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 37 citations in OpenAlex.
- Low-temperature magnetic resonance imaging with 2.8 μm isotropic resolution.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2018Article
- 3D printed microchannels for sub-nL NMR spectroscopy.PloS one · 2018Article
- NMR spectroscopy of single sub-nL ova with inductive ultra-compact single-chip probes.Scientific reports · 2017Article
- Investigation of the subcellular architecture of L7 neurons of Aplysia californica using magnetic resonance microscopy (MRM) at 7.8 microns.Scientific reports · 2015Article
- Review of methods to probe single cell metabolism and bioenergetics.Metabolic engineering · 2015Review
- Subcellular metabolite and lipid analysis of Xenopus laevis eggs by LAESI mass spectrometry.PloS one · 2014Article
- The Xenopus oocyte: a single-cell model for studying Ca2+ signaling.Cold Spring Harbor protocols · 2013Article
- Profiling metabolites and peptides in single cells.Nature methods · 2011Review
- NMR separation of intra- and extracellular compounds based on intermolecular coherences.Biophysical journal · 2010Article
- Article
- In vivo assessment of cold adaptation in insect larvae by magnetic resonance imaging and magnetic resonance spectroscopy.PloS one · 2008Article
- Lipid droplet motility and organelle contacts.Contact (Thousand Oaks (Ventura County, Calif.))Article
- Micron-scale magnetic resonance imaging based on low temperatures and dynamic nuclear polarization.Progress in nuclear magnetic resonance spectroscopyReview
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vivo magnetic resonance (MR) spectra are typically obtained from voxels whose spatial dimensions far exceed those of the cells they contain. This study was designed to evaluate the potential of localized MR spectroscopy to investigate subcellular phenomena. Using a high magnetic field and a home-built microscopy probe with large gradient field strengths, we achieved voxel sizes of (180 microm)3. In the large oocytes of the frog Xenopus laevis, this was small enough to allow the recording of the first compartment-selective in vivo MR spectra from the animal and vegetal cytoplasm as well as the nucleus. The two cytoplasmic regions differed in their lipid contents and NMR lineshape characteristics-differences that are not detectable with whole-cell NMR techniques. In the nucleus, the signal appeared to be dominated by water, whereas other contributions were negligible. We also used localized spectroscopy to monitor the uptake of diminazene acturate, an antitrypanosomal agent, into compartments of a single living oocyte. The resulting spectra from the nucleus and cytoplasm revealed different uptake kinetics for the two components of the drug and demonstrate that MR technology is on the verge of becoming a tool for cell biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.