ArticleBiophysical journal2006
Correlated fluorescence-atomic force microscopy of membrane domains: structure of fluorescence probes determines lipid localization.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 180 citations in OpenAlex.
- Quantification of lipid sorting during clathrin-mediated endocytosis.Nature cell biology · 2026Article
- Visualizing suborganellar lipid distribution using correlative light and electron microscopy.Nature cell biology · 2026Article
- Seeing lipids where they live.Nature cell biology · 2026Article
- Review
- Visualization of accessible cholesterol using a GRAM domain-based biosensor.Nature communications · 2023Article
- Eukaryotic Cell Membranes: Structure, Composition, Research Methods and Computational Modelling.International journal of molecular sciences · 2023Review
- Fluorescence strategies for mapping cell membrane dynamics and structures.APL bioengineering · 2020Article
- Raman Spectroscopy Study of Curvature-Mediated Lipid Packing and Sorting in Single Lipid Vesicles.Biophysical journal · 2019Article
- Sphingolipids and lipid rafts: Novel concepts and methods of analysis.Chemistry and physics of lipids · 2018Review
- Toxic HypF-N Oligomers Selectively Bind the Plasma Membrane to Impair Cell Adhesion Capability.Biophysical journal · 2018Article
- Characterization of Plasma Membrane Ceramides by Super-Resolution Microscopy.Angewandte Chemie (International ed. in English) · 2017Article
- Bright and photostable push-pull pyrene dye visualizes lipid order variation between plasma and intracellular membranes.Scientific reports · 2016Article
- Sphingolipid Organization in the Plasma Membrane and the Mechanisms That Influence It.Frontiers in cell and developmental biology · 2016Review
- Sphingomyelin distribution in lipid rafts of artificial monolayer membranes visualized by Raman microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Hemagglutinin clusters in the plasma membrane are not enriched with cholesterol and sphingolipids.Biophysical journal · 2015Article
- Fluorescent Sterols and Cholesteryl Esters as Probes for Intracellular Cholesterol Transport.Lipid insights · 2015Review
- Cholesterol stabilizes fluid phosphoinositide domains.Chemistry and physics of lipids · 2014Article
- Ultrafast diffusion of a fluorescent cholesterol analog in compartmentalized plasma membranes.Traffic (Copenhagen, Denmark) · 2014Article
- Inward cholesterol gradient of the membrane system in P. falciparum-infected erythrocytes involves a dilution effect from parasite-produced lipids.Biology open · 2014Article
- Membrane orientation and lateral diffusion of BODIPY-cholesterol as a function of probe structure.Biophysical journal · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coupling atomic force microscopy (AFM) with high-resolution fluorescence microscopy is an attractive means of identifying membrane domains by both physical topography and fluorescence. We have used this approach to study the ability of a suite of fluorescent molecules to probe domain structures in supported planar bilayers. These included BODIPY-labeled ganglioside, sphingomyelin, and three new cholesterol derivatives, as well as NBD-labeled phosphatidylcholine, sphingomyelin, and cholesterol. Interestingly, many fluorescent lipid probes, including derivatives of known raft-associated lipids, preferentially partitioned into topographical features consistent with nonraft domains. This suggests that the covalent attachment of a small fluorophore to a lipid molecule can abolish its ability to associate with rafts. In addition, the localization of one of the BODIPY-cholesterol derivatives was dependent on the lipid composition of the bilayer. These data suggest that conclusions about the identification of membrane domains in supported planar bilayers on the basis of fluorescent lipid probes alone must be interpreted with caution. The combination of AFM with fluorescence microscopy represents a more rigorous means of identifying lipid domains in supported bilayers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.