ReviewAdvances in clinical chemistry2005
Advances in real-time PCR: application to clinical laboratory diagnostics.
Review in Advances in clinical chemistry, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
49 citing papers in PubMed, 161 citations in OpenAlex.
- Cancer-associated fusion transcripts: mechanisms, functional roles, and clinical implications.Clinical and experimental medicine · 2026Review
- A decision-making process to guide the potential application of a hepatitis A virus and parvovirus B19 nucleic acid test for qualifying plasma for fractionation.Journal of the Association of Medical Microbiology and Infectious Disease Canada = Journal officiel de l'Association pour la microbiologie medicale et l'infectiologie Canada · 2026Article
- Awareness and Use of Home-Based Respiratory Pathogen Testing Services in the Internet Era: Postpandemic Questionnaire Study.JMIR formative research · 2026Article
- Cas12a Cis-cleavage mediated lateral flow assay enables multiplex and ultra-specific nucleic acid detection.Nature communications · 2025Article
- An open source, PCR based, point-of-care testing platform.Scientific reports · 2025Article
- Design and assessment of a multiplex real-time PCR method for simultaneous detection and differentiation of COVID-19 and Influenza A/B.Iranian journal of microbiology · 2025Article
- Development and Assessment of a Multiple-Analysis System for Diagnosing Malaria and Other Blood Parasite Infections in Humans and Non-Human Primates.Diagnostics (Basel, Switzerland) · 2025Article
- TRP36-ELISA forHeliyon · 2024Article
- Dual-Emission Fluorescence Resonance Energy Transfer (FRET) PCR DiscriminatesMicroorganisms · 2024Article
- Tackling Infectious Diseases with Rapid Molecular Diagnosis and Innovative Prevention.Infectious disease reports · 2024Review
- Article
- Reducing Bias and Quantifying Uncertainty in Fluorescence Produced by PCR.Bulletin of mathematical biology · 2023Article
- Adaptive Filtering Framework to Remove Nonspecific and Low-Efficiency Reactions in Multiplex Digital PCR Based on Sigmoidal Trends.Analytical chemistry · 2022Article
- Biosensors, modern technology for the detection of cancer-associated bacteria.Biotechnology letters · 2022Review
- Developing novel liquid biopsy by selective capture of viral RNA on magnetic beads to detect COVID-19.Iranian journal of basic medical sciences · 2022Article
- Highly multiplex PCR assays by coupling the 5'-flap endonuclease activity ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Penetration of the SARS-CoV-2 Spike Protein across the Blood-Brain Barrier, as Revealed by a Combination of a Human Cell Culture Model System and Optical Biosensing.Biomedicines · 2022Article
- An approach to quantitate maternal transcripts localized in sea urchin egg cortex using RT-qPCR with accurate normalization.PloS one · 2022Article
- Two Leishmania species separation targeting the ITS-rDNA and Cyt b genes by developing and evaluating HRM- qPCR.Revista da Sociedade Brasileira de Medicina Tropical · 2022Article
- Fast, accurate, point-of-care COVID-19 pandemic diagnosis enabled through advanced lab-on-chip optical biosensors: Opportunities and challenges.Applied physics reviews · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The polymerase chain reaction (PCR) has become one of the most important tools in molecular diagnostics, providing exquisite sensitivity and specificity for detection of nucleic acid targets. Real-time monitoring of PCR has simplified and accelerated PCR laboratory procedures and has increased information obtained from specimens including routine quantification and differentiation of amplification products. Clinical diagnostic applications and uses of real-time PCR are growing exponentially, real-time PCR is rapidly replacing traditional PCR, and new diagnostic uses likely will emerge. This review analyzes the scope of present and potential future clinical diagnostic applications of this powerful technique. Critical discussions focus on basic concepts, variations, data analysis, instrument platforms, signal detection formats, sample collection, assay design, and execution of real-time PCR.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.