ArticleProceedings of the National Academy of Sciences of the United States of America2005
Microarray-based detection of genetic heterogeneity, antimicrobial resistance, and the viable but nonculturable state in human pathogenic Vibrio spp.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed.
- Molecular Diagnostics of PathogenicInternational journal of molecular sciences · 2026Review
- The Effect of Non-Thermal Processing on the Fate of Pathogenic Bacteria and Hidden Hazardous Risks.Foods (Basel, Switzerland) · 2025Review
- Modeling pH and Temperature Effects as Climatic Hazards in VGeoHealth · 2023Article
- The Viable but Non-Culturable (VBNC) State in Vibrio Species: Why Studying the VBNC State Now Is More Exciting than Ever.Advances in experimental medicine and biology · 2023Article
- Multilocus Sequence Typing and Virulence Potential ofMicrobiology spectrum · 2022Article
- Article
- OMPdb: A Global Hub of Beta-Barrel Outer Membrane Proteins.Frontiers in bioinformatics · 2021Article
- Optimization of resuscitation-promoting broths for the revival ofFood science and biotechnology · 2021Article
- An Improved Approach to Identify Bacterial Pathogens to Human in Environmental Metagenome.Journal of microbiology and biotechnology · 2020Article
- Structural and Proteomic Changes in Viable but Non-culturableFrontiers in microbiology · 2019Article
- Article
- A European epidemiological survey of Vibrio splendidus clade shows unexplored diversity and massive exchange of virulence factors.World journal of microbiology & biotechnology · 2015Article
- Non-O1/non-O139 Vibrio cholerae carrying multiple virulence factors and V. cholerae O1 in the Chesapeake Bay, Maryland.Applied and environmental microbiology · 2015Article
- Insights into the environmental reservoir of pathogenic Vibrio parahaemolyticus using comparative genomics.Frontiers in microbiology · 2015Article
- Induction, resuscitation and quantitative real-time polymerase chain reaction analyses of viable but nonculturable Vibrio vulnificus in artificial sea water.World journal of microbiology & biotechnology · 2014Article
- The importance of the viable but non-culturable state in human bacterial pathogens.Frontiers in microbiology · 2014Review
- Genomic and phenotypic characterization of Vibrio cholerae non-O1 isolates from a US Gulf Coast cholera outbreak.PloS one · 2014Article
- Fitness factors in vibrios: a mini-review.Microbial ecology · 2013Review
- Single cell analysis of Vibrio harveyi uncovers functional heterogeneity in response to quorum sensing signals.BMC microbiology · 2012Article
- Vibrio cholerae in an Historically Cholera-Free Country.Environmental microbiology reports · 2012Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The morbidity and mortality associated with Vibrio-mediated waterborne diseases necessitates the development of sensitive detection technologies that are able to elucidate the identity, potential pathogenicity, susceptibility, and viability of contaminating bacteria in a timely manner. For this purpose, we have designed a single multiplex PCR assay to simultaneously amplify 95 diagnostic regions (encompassing species/serogroup-specific, antimicrobial resistance, and known toxin markers) and combined it with a long oligonucleotide microarray to create a platform capable of rapidly detecting and discriminating the major human pathogenic species from the genus Vibrio: V. cholerae, V. parahaemolyticus, V. vulnificus, and V. mimicus. We were able to validate this strategy by testing 100 geographically and temporally distributed isolates and observed an excellent concordance between species- and serotype-level microarray-based identification and traditional typing methods. In addition to accurate identification, the microarray simultaneously provided evidence of antibiotic resistance genes and mobile genetic elements, such as sulfamethoxazole-trimethoprim constins and class I integrons, and common toxin (ctxAB, rtxA, hap, hlyA, tl, tdh, trh, vvhA, vlly, and vmhA) and pathogenicity (tcpA, type III secretion system) genes that are associated with pathogenic Vibrio. The versatility of this method was further underscored by its ability to detect the expression of known toxin and virulence genes from potentially harmful viable but nonculturable organisms. The results suggest that this molecular identification method provides rapid and definitive information that would be of value in epidemiological, environmental, and health risk assessment surveillance.
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