ArticleMolecular and cellular biology2006
Rhp51-dependent recombination intermediates that do not generate checkpoint signal are accumulated in Schizosaccharomyces pombe rad60 and smc5/6 mutants after release from replication arrest.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 50 citations in OpenAlex.
- NFATC2IP is a mediator of SUMO-dependent genome integrity.Genes & development · 2024Article
- Brc1 Promotes the Focal Accumulation and SUMO Ligase Activity of Smc5-Smc6 during Replication Stress.Molecular and cellular biology · 2019Article
- SMC5/6: Multifunctional Player in Replication.Genes · 2018Review
- Specialized interfaces of Smc5/6 control hinge stability and DNA association.Nature communications · 2017Article
- Non-SMC Element 2 (NSMCE2) of the SMC5/6 Complex Helps to Resolve Topological Stress.International journal of molecular sciences · 2016Article
- Functional Crosstalk between the PP2A and SUMO Pathways Revealed by Analysis of STUbL Suppressor, razor 1-1.PLoS genetics · 2016Article
- Heterochromatic breaks move to the nuclear periphery to continue recombinational repair.Nature cell biology · 2015Article
- DNA repair. Proteomics reveals dynamic assembly of repair complexes during bypass of DNA cross-links.Science (New York, N.Y.) · 2015Article
- Checkpoints are blind to replication restart and recombination intermediates that result in gross chromosomal rearrangements.Nature communications · 2015Article
- Rad51-dependent aberrant chromosome structures at telomeres and ribosomal DNA activate the spindle assembly checkpoint.Molecular and cellular biology · 2014Article
- Involvement of Schizosaccharomyces pombe rrp1+ and rrp2+ in the Srs2- and Swi5/Sfr1-dependent pathway in response to DNA damage and replication inhibition.Nucleic acids research · 2013Article
- Fission yeast RecQ helicase Rqh1 is required for the maintenance of circular chromosomes.Molecular and cellular biology · 2013Article
- The Smc5/Smc6/MAGE complex confers resistance to caffeine and genotoxic stress in Drosophila melanogaster.PloS one · 2013Article
- DNA topoisomerase III localizes to centromeres and affects centromeric CENP-A levels in fission yeast.PLoS genetics · 2013Article
- Continued DNA synthesis in replication checkpoint mutants leads to fork collapse.Molecular and cellular biology · 2012Article
- Meiotic DNA joint molecule resolution depends on Nse5-Nse6 of the Smc5-Smc6 holocomplex.Nucleic acids research · 2012Article
- During replication stress, non-SMC element 5 (NSE5) is required for Smc5/6 protein complex functionality at stalled forks.The Journal of biological chemistry · 2012Article
- DNA repair and global sumoylation are regulated by distinct Ubc9 noncovalent complexes.Molecular and cellular biology · 2011Article
- Roles of vertebrate Smc5 in sister chromatid cohesion and homologous recombinational repair.Molecular and cellular biology · 2011Article
- Holliday junction-containing DNA structures persist in cells lacking Sgs1 or Top3 following exposure to DNA damage.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Schizosaccharomyces pombe rad60 gene is essential for cell growth and is involved in repairing DNA double-strand breaks. Rad60 physically interacts with and is functionally related to the structural maintenance of chromosomes 5 and 6 (SMC5/6) protein complex. In this study, we investigated the role of Rad60 in the recovery from the arrest of DNA replication induced by hydroxyurea (HU). rad60-1 mutant cells arrested mitosis normally when treated with HU. Significantly, Rad60 function is not required during HU arrest but is required on release. However, the mutant cells underwent aberrant mitosis accompanied by irregular segregation of chromosomes, and DNA replication was not completed, as revealed by pulsed-field gel electrophoresis. The deletion of rhp51 suppressed the aberrant mitosis of rad60-1 cells and caused mitotic arrest. These results suggest that Rhp51 and Rad60 are required for the restoration of a stalled or collapsed replication fork after release from the arrest of DNA replication by HU. The rad60-1 mutant was proficient in Rhp51 focus formation after release from the HU-induced arrest of DNA replication or DNA-damaging treatment. Furthermore, the lethality of a rad60-1 rqh1Delta double mutant was suppressed by the deletion of rhp51 or rhp57. These results suggest that Rad60 is required for recombination repair at a step downstream of Rhp51. We propose that Rhp51-dependent DNA structures that cannot activate the mitotic checkpoints accumulate in rad60-1 cells.
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