ArticleThe Journal of biological chemistry2006
Rational design of intercellular adhesion molecule-1 (ICAM-1) variants for antagonizing integrin lymphocyte function-associated antigen-1-dependent adhesion.
Article in The Journal of biological chemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 58 citations in OpenAlex.
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- Flex ddG: Rosetta Ensemble-Based Estimation of Changes in Protein-Protein Binding Affinity upon Mutation.The journal of physical chemistry. B · 2018Article
- Ligand- and cation-induced structural alterations of the leukocyte integrin LFA-1.The Journal of biological chemistry · 2018Article
- How special is the biochemical function of native proteins?F1000Research · 2016Review
- On the role of physics and evolution in dictating protein structure and function.Israel journal of chemistry · 2014Article
- Interplay of physics and evolution in the likely origin of protein biochemical function.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Applying physics-based scoring to calculate free energies of binding for single amino acid mutations in protein-protein complexes.PloS one · 2013Article
- Increasing affinity of interferon-γ receptor 1 to interferon-γ by computer-aided design.BioMed research international · 2013Article
- The C-terminal αI domain linker as a critical structural element in the conformational activation of αI integrins.The Journal of biological chemistry · 2011Article
- Regulation of integrin affinity on cell surfaces.The EMBO journal · 2011Article
- Anchored design of protein-protein interfaces.PloS one · 2011Article
- Engineering of single Ig superfamily domain of intercellular adhesion molecule 1 (ICAM-1) for native fold and function.The Journal of biological chemistry · 2010Article
- The protein-protein interface evolution acts in a similar way to antibody affinity maturation.The Journal of biological chemistry · 2010Article
- Computer-aided design of functional protein interactions.Nature chemical biology · 2009Review
- Design, expression and characterization of mutants of fasciculin optimized for interaction with its target, acetylcholinesterase.Protein engineering, design & selection : PEDS · 2009Article
- Structure-based design of a T-cell receptor leads to nearly 100-fold improvement in binding affinity for pepMHC.Proteins · 2009Article
- Computational design of antibody-affinity improvement beyond in vivo maturation.Nature biotechnology · 2007Article
- Structure-based protocol for identifying mutations that enhance protein-protein binding affinities.Journal of molecular biology · 2007Article
- Progress in computational protein design.Current opinion in biotechnology · 2007Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The interaction between integrin lymphocyte function-associated antigen-1 (LFA-1) and its ligand intercellular adhesion molecule-1 (ICAM-1) is critical in immunological and inflammatory reactions but, like other adhesive interactions, is of low affinity. Here, multiple rational design methods were used to engineer ICAM-1 mutants with enhanced affinity for LFA-1. Five amino acid substitutions 1) enhance the hydrophobicity and packing of residues surrounding Glu-34 of ICAM-1, which coordinates to a Mg2+ in the LFA-1 I domain, and 2) alter associations at the edges of the binding interface. The affinity of the most improved ICAM-1 mutant for intermediate- and high-affinity LFA-1 I domains was increased by 19-fold and 22-fold, respectively, relative to wild type. Moreover, potency was similarly enhanced for inhibition of LFA-1-dependent ligand binding and cell adhesion. Thus, rational design can be used to engineer novel adhesion molecules with high monomeric affinity; furthermore, the ICAM-1 mutant holds promise for targeting LFA-1-ICAM-1 interaction for biological studies and therapeutic purposes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.