ArticleJournal of bacteriology2006
Hfq is a regulator of F-plasmid TraJ and TraM synthesis in Escherichia coli.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Comprehensive analysis of Enterobacteriaceae IncX plasmids reveals robust conjugation regulators PrfaH, H-NS, and conjugation-fitness tradeoff.Communications biology · 2025Article
- A chromosomal mutation is superior to a plasmid-encoded mutation for plasmid fitness cost compensation.PLoS biology · 2024Article
- Small RNA GadY inFrontiers in cellular and infection microbiology · 2024Article
- Microbial applications for sustainable space exploration beyond low Earth orbit.NPJ microgravity · 2023Review
- Natural and Artificial Strategies To Control the Conjugative Transmission of Plasmids.Microbiology spectrum · 2018Review
- DNA-Binding Proteins Regulating pIP501 Transfer and Replication.Frontiers in molecular biosciences · 2016Review
- Small RNA-based feedforward loop with AND-gate logic regulates extrachromosomal DNA transfer in Salmonella.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Crystal structures of the F and pSLT plasmid TraJ N-terminal regions reveal similar homodimeric PAS folds with functional interchangeability.Biochemistry · 2014Article
- TraK and TraB are conserved outer membrane proteins of the Neisseria gonorrhoeae Type IV secretion system and are expressed at low levels in wild-type cells.Journal of bacteriology · 2014Article
- Error-prone PCR mutagenesis reveals functional domains of a bacterial transcriptional activator, TraJ.Journal of bacteriology · 2012Article
- Hfq and its constellation of RNA.Nature reviews. Microbiology · 2011Review
- Characterization and global gene expression of F- phenocopies during Escherichia coli biofilm formation.Molecular genetics and genomics : MGG · 2010Article
- Increased expression of the type IV secretion system in piliated Neisseria gonorrhoeae variants.Journal of bacteriology · 2010Article
- Regulation of bacterial conjugation in microaerobiosis by host-encoded functions ArcAB and sdhABCD.Genetics · 2010Article
- TraR, a homolog of a RNAP secondary channel interactor, modulates transcription.PLoS genetics · 2009Article
- Conjugal transfer of the Salmonella enterica virulence plasmid in the mouse intestine.Journal of bacteriology · 2008Article
- Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- GroEL plays a central role in stress-induced negative regulation of bacterial conjugation by promoting proteolytic degradation of the activator protein TraJ.Journal of bacteriology · 2007Article
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Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The F plasmid of Escherichia coli allows horizontal DNA transfer between an F(+) donor cell and an F(-) recipient. Expression of the transfer genes is tightly controlled by a number of factors, including the following plasmid-encoded regulatory proteins: TraJ, the primary activator of the 33-kb tra operon, and the autoregulators TraM and TraY. Here, we demonstrate that the host RNA binding protein, Hfq, represses TraJ and TraM synthesis by destabilizing their respective mRNAs. Mating assays and immunoblot analyses for TraM and TraJ showed that transfer efficiency and protein levels increased in host cells containing a disruption in hfq compared to wild-type cells in stationary phase. The stability of transcripts containing a putative Hfq binding site located in the intergenic untranslated region between traM and traJ was increased in hfq mutant donor cells, suggesting that Hfq destabilizes these transcripts. Electrophoretic mobility shift assays demonstrated that Hfq specifically binds this region but not the antisense RNA, FinP, encoded on the opposite strand. Together, these findings indicate that Hfq regulates traM and traJ transcript stability by a mechanism separate from FinOP-mediated repression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.