ArticleJournal of bacteriology2006
Identification and characterization of the genes involved in glycosylation pathways of mycobacterial glycopeptidolipid biosynthesis.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Functional characterization of GplA (MSMEG-0394) reveals a novel determinant of glycopeptidolipids homeostasis inFrontiers in microbiology · 2026Article
- Identification of an uncharacterized protein as a novel regulator ofJournal of virology · 2025Article
- Insertion Mutation ofInfection and drug resistance · 2022Article
- Biochemical characterization of a glycosyltransferase Gtf3 from3 Biotech · 2020Article
- Glycopeptidolipid of Mycobacterium smegmatis J15cs Affects Morphology and Survival in Host Cells.PloS one · 2015Article
- A GMC oxidoreductase homologue is required for acetylation of glycopeptidolipid in Mycobacterium smegmatis.Biochemistry · 2014Article
- Rough colony morphology of Mycobacterium massiliense Type II genotype is due to the deletion of glycopeptidolipid locus within its genome.BMC genomics · 2013Article
- Retrobiosynthetic approach delineates the biosynthetic pathway and the structure of the acyl chain of mycobacterial glycopeptidolipids.The Journal of biological chemistry · 2012Article
- Methylation--an uncommon modification of glycans.Biological chemistry · 2012Review
- Biofilm formation by Mycobacterium avium isolates originating from humans, swine and birds.BMC microbiology · 2009Article
- Defining mycobacteria: Shared and specific genome features for different lifestyles.Indian journal of microbiology · 2009Article
- The Mycobacterium avium complex gtfTB gene encodes a glucosyltransferase required for the biosynthesis of serovar 8-specific glycopeptidolipid.Journal of bacteriology · 2008Article
- The mycobacterial glycopeptidolipids: structure, function, and their role in pathogenesis.Glycobiology · 2008Review
- Structural analysis and biosynthesis gene cluster of an antigenic glycopeptidolipid from Mycobacterium intracellulare.Journal of bacteriology · 2008Article
- Identification and characterization of two novel methyltransferase genes that determine the serotype 12-specific structure of glycopeptidolipids of Mycobacterium intracellulare.Journal of bacteriology · 2008Article
- Characterization of the fucosylation pathway in the biosynthesis of glycopeptidolipids from Mycobacterium avium complex.Journal of bacteriology · 2007Article
- Genomics of glycopeptidolipid biosynthesis in Mycobacterium abscessus and M. chelonae.BMC genomics · 2007Article
- Structural characterization of a specific glycopeptidolipid containing a novel N-acyl-deoxy sugar from mycobacterium intracellulare serotype 7 and genetic analysis of its glycosylation pathway.Journal of bacteriology · 2007Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycopeptidolipids (GPLs) are major components present on the outer layers of the cell walls of several nontuberculous mycobacteria. GPLs are antigenic molecules and have variant oligosaccharides in mycobacteria such as Mycobacterium avium. In this study, we identified four genes (gtf1, gtf2, gtf3, and gtf4) in the genome of Mycobacterium smegmatis. These genes were independently inactivated by homologous recombination in M. smegmatis, and the structures of GPLs from each gene disruptant were analyzed. Thin-layer chromatography, gas chromatography-mass spectrometry, and matrix-assisted laser desorption ionization-time-of-flight mass spectrometry analyses revealed that the mutants Deltagtf1 and Deltagtf2 accumulated the fatty acyl-tetrapeptide core having O-methyl-rhamnose and 6-deoxy-talose as sugar residues, respectively. The mutant Deltagtf4 possessed the same GPLs as the wild type, whereas the mutant Deltagtf3 lacked two minor GPLs, consisting of 3-O-methyl-rhamnose attached to O-methyl-rhamnose of the fatty acyl-tetrapeptide core. These results indicate that the gtf1 and gtf2 genes are responsible for the early glycosylation steps of GPL biosynthesis and the gtf3 gene is involved in transferring a rhamnose residue not to 6-deoxy-talose but to an O-methyl-rhamnose residue. Moreover, a complementation experiment showed that M. avium gtfA and gtfB, which are deduced glycosyltransferase genes of GPL biosynthesis, restore complete GPL production in the mutants Deltagtf1 and Deltagtf2, respectively. Our findings propose that both M. smegmatis and M. avium have the common glycosylation pathway in the early steps of GPL biosynthesis but differ at the later stages.
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