ArticleBMC biology2005
Increased synaptic microtubules and altered synapse development in Drosophila sec8 mutants.
Article in BMC biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Altered methylation pattern in EXOC4 is associated with stroke outcome: an epigenome-wide association study.Clinical epigenetics · 2022Pooled it
- Sec8: a novel positive regulator of RIG-I in anti-RNA viral defense.Cell death & disease · 2026Article
- Unveiling EXOC4/SEC8: a key player in enhancing antiviral immunity by inhibiting the FBXL19-STING1-SQSTM1 signaling axis.Autophagy · 2025Article
- Inhibition of the Exocyst Complex Attenuates the LRRK2 Pathological Effects.International journal of molecular sciences · 2023Article
- Secreted Amyloid Precursor Protein Alpha, a Neuroprotective Protein in the Brain Has Widespread Effects on the Transcriptome and Proteome of Human Inducible Pluripotent Stem Cell-Derived Glutamatergic Neurons Related to Memory Mechanisms.Frontiers in neuroscience · 2022Article
- Article
- The Presynaptic Microtubule Cytoskeleton in Physiological and Pathological Conditions: Lessons from Drosophila Fragile X Syndrome and Hereditary Spastic Paraplegias.Frontiers in molecular neuroscience · 2016Review
- The Extracellular-Regulated Kinase Effector Lk6 is Required for Glutamate Receptor Localization at the Drosophila Neuromuscular Junction.Journal of experimental neuroscience · 2016Article
- Prodomain removal enables neto to stabilize glutamate receptors at the Drosophila neuromuscular junction.PLoS genetics · 2015Article
- Staufen targets coracle mRNA to Drosophila neuromuscular junctions and regulates GluRIIA synaptic accumulation and bouton number.Developmental biology · 2014Article
- Ral mediates activity-dependent growth of postsynaptic membranes via recruitment of the exocyst.The EMBO journal · 2013Article
- A novel strategy to isolate ubiquitin conjugates reveals wide role for ubiquitination during neural development.Molecular & cellular proteomics : MCP · 2011Article
- The Cdc42-selective GAP rich regulates postsynaptic development and retrograde BMP transsynaptic signaling.The Journal of cell biology · 2010Article
- Article
- A Syd-1 homologue regulates pre- and postsynaptic maturation in Drosophila.The Journal of cell biology · 2010Article
- Sec3-containing exocyst complex is required for desmosome assembly in mammalian epithelial cells.Molecular biology of the cell · 2010Article
- Regulation of glutamate receptor subunit availability by microRNAs.The Journal of cell biology · 2009Article
- Effect of ambient extracellular glutamate on Drosophila glutamate receptor trafficking and function.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2009Article
- Identification and investigation of Drosophila postsynaptic density homologs.Bioinformatics and biology insights · 2008Article
- Formin-dependent synaptic growth: evidence that Dlar signals via Diaphanous to modulate synaptic actin and dynamic pioneer microtubules.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSec8 is highly expressed in mammalian nervous systems and has been proposed to play a role in several aspects of neural development and function, including neurite outgrowth, calcium-dependent neurotransmitter secretion, trafficking of ionotropic glutamate receptors and regulation of neuronal microtubule assembly. However, these models have never been tested in vivo. Nervous system development and function have not been described after mutation of sec8 in any organism.
resultsWe identified lethal sec8 mutants in an unbiased forward genetic screen for mutations causing defects in development of glutamatergic Drosophila neuromuscular junctions (NMJs). The Drosophila NMJ is genetically malleable and accessible throughout development to electrophysiology and immunocytochemistry, making it ideal for examination of the sec8 mutant synaptic phenotype. We developed antibodies to Drosophila Sec8 and showed that Sec8 is abundant at the NMJ. In our sec8 null mutants, in which the sec8 gene is specifically deleted, Sec8 immunoreactivity at the NMJ is eliminated but immunoblots reveal substantial maternal contribution in the rest of the animal. Contrary to the hypothesis that Sec8 is required for neurite outgrowth or synaptic terminal growth, immunocytochemical examination revealed that sec8 mutant NMJs developed more branches and presynaptic terminals during larval development, compared to controls. Synaptic electrophysiology showed no evidence that Sec8 is required for basal neurotransmission, though glutamate receptor trafficking was mildly disrupted in sec8 mutants. The most dramatic NMJ phenotype in sec8 mutants was an increase in synaptic microtubule density, which was approximately doubled compared to controls.
conclusionSec8 is abundant in the Drosophila NMJ. Sec8 is required in vivo for regulation of synaptic microtubule formation, and (probably secondarily) regulation of synaptic growth and glutamate receptor trafficking. We did not find any evidence that Sec8 is required for basal neurotransmission.
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