ArticleApplied and environmental microbiology1998
Laser microsurgery permits fungal plasma membrane single-ion-channel resolution at the hyphal tip.
Article in Applied and environmental microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
5 citing papers in PubMed, 19 citations in OpenAlex.
- In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi.Microsystems & nanoengineering · 2024Article
- ATP modulation of osmotically activated anionic current in the membrane of Phycomyces blakesleeanus sporangiophore.Scientific reports · 2023Article
- Outwardly rectifying anionic channel from the plasma membrane of the fungus Phycomyces blakesleeanus.Eukaryotic cell · 2009Article
- TOK homologue in Neurospora crassa: first cloning and functional characterization of an ion channel in a filamentous fungus.Eukaryotic cell · 2003Article
- Hyperpolarization-activated calcium channels at the tip of Arabidopsis root hairs.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A method for formation of high-electrical-resistance seals on the Neurospora crassa plasma membrane, allowing resolution of single-ion-channel activity by patch clamp electrophysiology, is reported. Laser microsurgery permits access to the hyphal apex without enzymatic cell wall digestion and loss of morphological polarity. Cell wall reformation is delayed by brefeldin. This method can allow full characterization of apical plasma membrane channels, which are implicated in tip growth.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.