ArticleThe Journal of general physiology2006
Effects of multiple metal binding sites on calcium and magnesium-dependent activation of BK channels.
Article in The Journal of general physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Magnesium Ions as Modulators of Voltage-Gated and Ligand-Gated Ion Channels in Central Neurons.International journal of molecular sciences · 2025Review
- Coupling of CaProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Interactions of divalent cations with calcium binding sites of BK channels reveal independent motions within the gating ring.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Ethanol Effect on BK Channels is Modulated by Magnesium.Alcoholism, clinical and experimental research · 2015Article
- Transduction of voltage and Ca2+ signals by Slo1 BK channels.Physiology (Bethesda, Md.) · 2013Review
- State-dependent FRET reports calcium- and voltage-dependent gating-ring motions in BK channels.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The BK channel: a vital link between cellular calcium and electrical signaling.Protein & cell · 2012Review
- Mg(2+) binding to open and closed states can activate BK channels provided that the voltage sensors are elevated.The Journal of general physiology · 2011Article
- Metal-driven operation of the human large-conductance voltage- and Ca2+-dependent potassium channel (BK) gating ring apparatus.The Journal of biological chemistry · 2011Article
- Activation of Slo1 BK channels by Mg2+ coordinated between the voltage sensor and RCK1 domains.Nature structural & molecular biology · 2008Article
- An extracellular Cu2+ binding site in the voltage sensor of BK and Shaker potassium channels.The Journal of general physiology · 2008Article
- Mg2+ enhances voltage sensor/gate coupling in BK channels.The Journal of general physiology · 2008Article
- Mg2+ mediates interaction between the voltage sensor and cytosolic domain to activate BK channels.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Tuning magnesium sensitivity of BK channels by mutations.Biophysical journal · 2006Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
BK channels are activated by physiological concentrations of intracellular Ca2+ and Mg2+ in a variety of cells. Previous studies have identified two sites important for high-affinity Ca2+ sensing between [Ca2+]i of 0.1-100 microM and a site important for Mg2+ sensing between [Mg2+]i of 0.1-10 mM. BK channels can be also activated by Ca2+ and Mg2+ at concentrations>10 mM so that the steady-state conductance and voltage (G-V) relation continuously shifts to more negative voltage ranges when [Mg2+]i increases from 0.1-100 mM. We demonstrate that a novel site is responsible for metal sensing at concentrations>=10 mM, and all four sites affect channel activation independently. As a result, the contributions of these sites to channel activation are complex, depending on the combination of Ca2+ and Mg2+ concentrations. Here we examined the effects of each of these sites on Ca2+ and Mg2+-dependent activation and the data are consistent with the suggestion that these sites are responsible for metal binding. We provide an allosteric model for quantitative estimation of the contributions that each of these putative binding sites makes to channel activation at any [Ca2+]i and [Mg2+]i.
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