Evidence map›Paper›PMID 16343936›Full record

ReviewMethods (San Diego, Calif.)2006

Imaging the division process in living tissue culture cells.

Alexey Khodjakov, Conly L Rieder

Abstract readReview
In one paragraph

Review in Methods (San Diego, Calif.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed.

  1. Article
  2. Article
  3. Near single-photon imaging in the shortwave infrared using homodyne detection.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  4. Article
  5. Dose-Extreme Mechanics Letters · 2021
    Article
  6. Imaging mitotic processes in three dimensions with lattice light-sheet microscopy.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021
    Review
  7. Article
  8. Article
  9. Visualizing the Early Stages of Phagocytosis.Journal of visualized experiments : JoVE · 2017
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Alexey KhodjakovWadsworth Center, New York State Department of Health, Albany, NY 12201-0509, USA. khodj@wadswoirth.org
Conly L Rieder

Funding

FUNCTIONAL PROPERTIES OF CENTROSOMES IN SOMATIC CELLSR01GM059363 · NIGMS · WADSWORTH CENTER · PI KHODJAKOV, ALEXEY L · 1999 to 2017
$6.3M
FORMATION,INTERACTION &FUNCTION OF SPINDLE COMPONENTSR37GM040198 · NIGMS · WADSWORTH CENTER · PI RIEDER, CONLY L. · 2001 to 2010
$3.9M
NIGMS NIH HHS R01 GM059363NIGMS NIH HHS R37 GM040198
6 · The paper itself

Abstract

We detail some of the pitfalls encountered when following live cultured somatic cells by light microscopy during mitosis. Principle difficulties in this methodology arise from the necessity to compromise between maintaining the health of the cell while achieving the appropriate temporal and spatial resolutions required for the study. Although the quality of the data collected from fixed cells is restricted only by the quality of the imaging system and the optical properties of the specimen, the major limiting factor when viewing live cells is radiation damage induced during illumination. We discuss practical considerations for minimizing this damage, and for maintaining the general health of the cell, while it is being followed by multi-mode or multi-dimensional light microscopy.

Indexed as

Cell Culture TechniquesLightAnimalsCell DivisionCells, CulturedImage EnhancementLensesMicroscopy, FluorescenceMicroscopy, VideoTemperature

Identifiers

PMID16343936
PMCPMC2590767

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.