ArticleThe anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology2006
In vivo delivery of fluoresceinated dextrans to the murine growth plate: imaging of three vascular routes by multiphoton microscopy.
Article in The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Weekly Lonapegsomatropin in Treatment-Naïve Children With Growth Hormone Deficiency: The Phase 3 heiGHt Trial.The Journal of clinical endocrinology and metabolism · 2021Trial
- Achondroplasia: Current Disease-Modifying Therapies and Genome Editing Strategies.International journal of molecular sciences · 2026Review
- Once-Weekly Lonapegsomatropin Was Efficacious and Well Tolerated in Chinese Children with Growth Hormone Deficiency: Results from a Phase 3 Randomized Trial.Hormone research in paediatrics · 2026Article
- Long-Acting Growth Hormone Preparations - Current Status and Future Considerations.The Journal of clinical endocrinology and metabolism · 2020Review
- The Actions of IGF-1 in the Growth Plate and Its Role in Postnatal Bone Elongation.Current osteoporosis reports · 2020Review
- Molecular Signaling Interactions and Transport at the Osteochondral Interface: A Review.Frontiers in cell and developmental biology · 2020Review
- Live imaging analysis of the growth plate in a murine long bone explanted culture system.Scientific reports · 2018Article
- Imaging IGF-I uptake in growth plate cartilage using in vivo multiphoton microscopy.Journal of applied physiology (Bethesda, Md. : 1985) · 2017Article
- The rationale and design of TransCon Growth Hormone for the treatment of growth hormone deficiency.Endocrine connections · 2017Review
- Stimulating Fracture Healing in Ischemic Environments: Does Oxygen Direct Stem Cell Fate during Fracture Healing?Frontiers in cell and developmental biology · 2017Review
- Hindlimb heating increases vascular access of large molecules to murine tibial growth plates measured by in vivo multiphoton imaging.Journal of applied physiology (Bethesda, Md. : 1985) · 2014Article
- The role of oxygen during fracture healing.Bone · 2013Article
- Site-1 protease is essential to growth plate maintenance and is a critical regulator of chondrocyte hypertrophic differentiation in postnatal mice.The Journal of biological chemistry · 2011Article
- Exercise mitigates the stunting effect of cold temperature on limb elongation in mice by increasing solute delivery to the growth plate.Journal of applied physiology (Bethesda, Md. : 1985) · 2010Article
- Temperature alters solute transport in growth plate cartilage measured by in vivo multiphoton microscopy.Journal of applied physiology (Bethesda, Md. : 1985) · 2009Article
- Solute transport in growth plate cartilage: in vitro and in vivo.Biophysical journal · 2007Article
- Analysis of the orientation of primary cilia in growth plate cartilage: a mathematical method based on multiphoton microscopical images.Journal of structural biology · 2007Article
- Metabolic consideration of epiphyseal growth: survival responses in a taxing environment.Bone · 2007Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Bone elongation by endochondral ossification occurs through the differentiation cascade of chondrocytes of cartilaginous growth plates. Molecules from the systemic vasculature reach the growth plate from three different directions: epiphyseal, metaphyseal, and a ring vessel and plexus associated with the perichondrium. This study is an analysis of the real-time dynamics of entrance of fluoresceinated tracers of different molecular weights into the growth plate from the systemic vasculature and tests the hypothesis that molecular weight is a key variable in the determination of both the directionality and the extent of tracer movement into the growth plate. Multiphoton microscopy was used for direct in vivo imaging of the murine proximal tibial growth plate in anesthetized 4- to 5-week-old transgenic mice with green fluorescent protein linked to the collagen II promoter. Mice were given an intracardiac injection of either fluorescein (332.3 Da) or fluoresceinated dextrans of 3, 10, 40, 70 kDa, singly or sequentially. For each tracer, directionality and rate of arrival, together with extent of movement within the growth plate, were imaged in real time. For small molecules (up to 10 kDa), vascular access from all three directions was observed and entrance was equally permissive from the metaphyseal and the epiphyseal sides. Within our detection limit (a few percent of vascular concentration), 40 kDa and larger dextrans did not enter. These results have implications both for understanding systemic and paracrine regulation of growth plate chondrocytic differentiation, as well as variables associated with effective drug delivery to growth plate chondrocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.