ArticleParasitology research2006
Cloning and analysis of a cDNA encoding a putative serine protease comprising two trypsin-like domains of Trichinella spiralis.
Article in Parasitology research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Application of a recombinant novel trypsin from Trichinella spiralis for serodiagnosis of trichinellosis.Parasites & vectors · 2024Article
- A Trichinella spiralis new born larvae-specific protein, Ts-NBL1, interacts with host's cell vimentin.Parasitology research · 2022Article
- Immunomodulatory components ofEXCLI journal · 2022Article
- Oral Vaccination of Mice WithFrontiers in microbiology · 2022Article
- Caenorhabditis elegans in anthelmintic research - Old model, new perspectives.International journal for parasitology. Drugs and drug resistance · 2020Review
- Molecular characterization of a 31 kDa protein from Trichinella spiralis and its induced immune protection in BALB/c mice.Parasites & vectors · 2018Article
- Identification of a host collagen inducing factor from the excretory secretory proteins of Trichinella spiralis.PLoS neglected tropical diseases · 2018Article
- Phylogenomic and biogeographic reconstruction of the Trichinella complex.Nature communications · 2016Article
- Serine proteases of parasitic helminths.The Korean journal of parasitology · 2015Review
- Identification of early diagnostic antigens from major excretory-secretory proteins of Trichinella spiralis muscle larvae using immunoproteomics.Parasites & vectors · 2014Article
- An antigenic recombinant serine protease from Trichinella spiralis induces protective immunity in BALB/c mice.Parasitology research · 2013Article
- Serine protease-mediated host invasion by the parasitic nematode Steinernema carpocapsae.The Journal of biological chemistry · 2010Article
- Role of enteric nerves in immune-mediated changes in protease-activated receptor 2 effects on gut function.Neurogastroenterology and motility · 2010Article
- Functional genes and proteins of Trichinella spp.Parasitology research · 2009Review
- Expressed sequence tags of Trichinella spiralis muscle stage larvae.The Korean journal of parasitology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cDNA encoding a putative serine protease, TsSerP, was cloned by degenerative polymerase chain reaction and screening of the cDNA library from Trichinella spiralis adult-newborn larvae stage. Sequence analysis revealed the presence of two trypsin-like serine protease domains flanking a hydrophilic domain, with the catalytic triad residue histidine in the alpha domain substituted by an arginine residue. Southern blots indicated that this was a single copy gene in the parasite genome. Northern blots demonstrated a single 2.3-kb transcript during the muscle larvae and adult stages of T. spiralis. The recombinant protein from the TsSerP beta domain (betaSerP) was produced but not recognised by T. spiralis-infected swine serum. An anti-betaSerP polyclonal serum detected a 69-kDa polypeptide in the soluble antigens of T. spiralis muscle larvae. Immunolocalisation analysis located TsSerP on the inner layer of the cuticle and oesophagus of the parasite, suggesting a potential role in its moulting and/or digestive functions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.