ArticlePlanta2006
Nitric oxide scavenging by barley hemoglobin is facilitated by a monodehydroascorbate reductase-mediated ascorbate reduction of methemoglobin.
Article in Planta, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 126 citations in OpenAlex.
- Modulation of GSNOR activity for improved NO homeostasis and flood resilience in plants.Plant signaling & behavior · 2026Review
- The role of alternative oxidase in the maintenance of cellular redox balance under hypoxia via participation in nitric oxide turnover.Journal of experimental botany · 2025Review
- The ascorbate-glutathione cycle coming of age.Journal of experimental botany · 2024Review
- Nitric Oxide and Globin Glb1 RegulateAntioxidants (Basel, Switzerland) · 2023Article
- Control of the rhizobium-legume symbiosis by the plant nitrogen demand is tightly integrated at the whole plant level and requires inter-organ systemic signaling.Frontiers in plant science · 2023Review
- Oxidative Implications of Substituting a Conserved Cysteine Residue in Sugar Beet Phytoglobin BvPgb 1.2.Antioxidants (Basel, Switzerland) · 2022Article
- Flooding Tolerance in Sweet Potato (Antioxidants (Basel, Switzerland) · 2022Article
- Expressed Soybean Leghemoglobin: Effect onMolecules (Basel, Switzerland) · 2021Article
- ROS and NO Regulation by Melatonin Under Abiotic Stress in Plants.Antioxidants (Basel, Switzerland) · 2020Review
- Tolerant mechanisms to OJournal of plant research · 2020Article
- Improving Air Quality by Nitric Oxide Consumption of Climate-Resilient Trees Suitable for Urban Greening.Frontiers in plant science · 2020Article
- Multilevel Regulation of Peroxisomal Proteome by Post-Translational Modifications.International journal of molecular sciences · 2019Review
- Phytoglobin overexpression promotes barley growth in the presence of enhanced level of atmospheric nitric oxide.Journal of experimental botany · 2019Article
- Nitrate nutrition influences multiple factors in order to increase energy efficiency under hypoxia in Arabidopsis.Annals of botany · 2019Article
- Analysis of potential strategies for cadmium stress tolerance revealed by transcriptome analysis of upland cotton.Scientific reports · 2019Article
- Regulation of the Central Carbon Metabolism in Apple Fruit Exposed to Postharvest Low-Oxygen Stress.Frontiers in plant science · 2019Review
- Comparative Analysis of the Nodule Transcriptomes ofFrontiers in plant science · 2018Article
- Characterization of the Heme Pocket Structure and Ligand Binding Kinetics of Non-symbiotic Hemoglobins from the Model LegumeFrontiers in plant science · 2017Article
- Phytoglobin expression influences soil flooding response of corn plants.Annals of botany · 2016Article
- Reactive Nitrogen Species in Mitochondria and Their Implications in Plant Energy Status and Hypoxic Stress Tolerance.Frontiers in plant science · 2016Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NADH-dependent NO scavenging in barley extracts is linked to hemoglobin (Hb) expression and is inhibited by SH-reagents. Barley Hb has a single cysteine residue. To determine whether this cysteine was critical for NO scavenging, barley Hb and a mutated version, in which the single Cys(79) was replaced by Ser, were over-expressed in Escherichia coli and purified to near homogeneity. The purified proteins exhibited very low NO-scavenging activity (12-14 nmol min(-1) mg(-1) protein) in the presence of NADH or NADPH. This activity was insensitive to SH-reagents. Addition of an extract from barley roots to either of the purified proteins resulted in high NADH-dependent NO turnover in a reaction that was sensitive to SH-reagents. A protein was purified from barley roots and identified by mass-spectrometry analysis as a cytosolic monodehydroascorbate reductase. It efficiently supported NADH-dependent NO scavenging in the presence of either native or mutated barley Hb. Ascorbate strongly facilitated the rate of metHb reduction. The K (m) for Hb was 0.3 microM, for ascorbate 0.6 mM and for NADH 4 microM. The reaction in the presence of monodehydroascorbate reductase was sensitive to SH-reagents with either form of the Hb. We conclude that metHb reduction and NO turnover do not involve direct participation of the Cys(79) residue of barley Hb. NO scavenging is facilitated by monodehydroascorbate reductase mediating a coupled reaction involving ferric Hb reduction in the presence of ascorbate and NADH.
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