ArticleAmerican journal of respiratory cell and molecular biology2006
Cholinergic receptor and cyclic stretch-mediated inflammatory gene expression in intact ASM.
Article in American journal of respiratory cell and molecular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 53 citations in OpenAlex.
- Pro-Inflammatory Response of Bovine Lung Explant Induced byPathogens (Basel, Switzerland) · 2026Article
- Long-Acting Muscarinic Antagonists for the Treatment of Difficult-to-Treat and Severe Asthma: A Narrative Review Focusing on Inflammation from Bench to Bedside.Journal of inflammation research · 2026Review
- The regulatory action of acetylcholine and its receptors on B4 and C4 leukotriene formation in the porcine endometrium after experimental inflammogenicJournal of veterinary research · 2024Article
- Mechanical Ventilation-Related High Stretch Mainly Induces Endoplasmic Reticulum Stress and Thus Mediates Inflammation Response in Cultured Human Primary Airway Smooth Muscle Cells.International journal of molecular sciences · 2023Article
- Muscarinic receptor antagonists and airway inflammation: A systematic review on pharmacological models.Heliyon · 2022Review
- Cholinergic System and Its Therapeutic Importance in Inflammation and Autoimmunity.Frontiers in immunology · 2021Review
- Targeting Mechano-Transcription Process as Therapeutic Intervention in Gastrointestinal Disorders.Frontiers in pharmacology · 2021Review
- Pro-Inflammatory Response of Bovine Polymorphonuclear Cells Induced byFrontiers in veterinary science · 2020Article
- Article
- Mechanical Regulation of Gene Expression in Gut Smooth Muscle Cells.Frontiers in physiology · 2017Review
- The impact of low-frequency, low-force cyclic stretching of human bronchi on airway responsiveness.Respiratory research · 2016Article
- Connexin 43 is overexpressed in human fetal membrane defects after fetoscopic surgery.Prenatal diagnosis · 2016Article
- Uterine overdistention induces preterm labor mediated by inflammation: observations in pregnant women and nonhuman primates.American journal of obstetrics and gynecology · 2015Article
- Long-acting muscarinic receptor antagonists for the treatment of chronic airway diseases.Therapeutic advances in chronic disease · 2014Review
- Mechanical stress is a pro-inflammatory stimulus in the gut: in vitro, in vivo and ex vivo evidence.PloS one · 2014Article
- Cellular mechanism of mechanotranscription in colonic smooth muscle cells.American journal of physiology. Gastrointestinal and liver physiology · 2012Article
- Prophylactic and therapeutic benefits of COX-2 inhibitor on motility dysfunction in bowel obstruction: roles of PGE₂ and EP receptors.American journal of physiology. Gastrointestinal and liver physiology · 2012Article
- Cholinergic regulation of airway inflammation and remodelling.Journal of allergy · 2012Article
- Length-dependent modulation of cytoskeletal remodeling and mechanical energetics in airway smooth muscle.American journal of respiratory cell and molecular biology · 2011Article
- Airway response to acute mechanical stress in a human bronchial model of stretch.Critical care (London, England) · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We tested the hypothesis that cholinergic stimulation and cyclic stretch regulate inflammatory gene expression in intact airway smooth muscle by measuring mRNA expression in bovine tracheal smooth muscle using limited microarray analysis and RT-PCR. Carbachol (1 microM) induced significant increases in the expression of cyclooxygenase (COX)-1, COX-2, IL-8, and plasminogen activator, urokinase type (PLAU) to levels ranging from 1.3- to 3.1-fold of control. Sinusoidal length oscillation at an amplitude of 10% muscle length and a frequency of 1 Hz induced significant increases in the expression of CCL-2, COX-2, IL-1 beta, and IL-6 to levels ranging from 12- to 206-fold of control. Decreasing the oscillatory amplitude by 50% did not significantly change inflammatory gene expression. In contrast, decreasing the oscillatory frequency by 50% significantly attenuated inflammatory gene expression by 76-93%. Nifedipine (1 microM) had an insignificant effect on carbachol-induced gene expression, but significantly inhibited sinusoidal length oscillation-induced inflammatory gene expression by 40-78%. Correlation analysis revealed two groups of genes with differential responses to sinusoidal length oscillation. The highly responsive group included COX-2, IL-6, and IL-8, which exhibited 45- to 364-fold increases in gene expression in response to sinusoidal length oscillation. The moderately responsive group included CCL2 and PLAU, which exhibited 13- to 19-fold increases in gene expression in response to sinusoidal oscillation. These findings suggest that cyclic stretch regulates inflammatory gene expression in intact airway smooth muscle in an amplitude- and frequency-dependent manner by modulating the activity of L-type voltage-gated calcium channels.
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Registered trials
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