ArticleBiophysical journal2006
Neuronal SNAREs do not trigger fusion between synthetic membranes but do promote PEG-mediated membrane fusion.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
78 citing papers in PubMed, 139 citations in OpenAlex.
- From Population Averaging to Single Event Resolution: Evolution of Sensing Platforms for Membrane Fusion.Sensors (Basel, Switzerland) · 2026Review
- SM protein Sly1 and a SNARE Habc domain promote membrane fusion through multiple mechanisms.The Journal of cell biology · 2024Article
- Serum Metabolomics of Benign Essential Blepharospasm Using Liquid Chromatography and Orbitrap Mass Spectrometry.Oxidative medicine and cellular longevity · 2022Article
- Local externalization of phosphatidylserine mediates developmental synaptic pruning by microglia.The EMBO journal · 2020Article
- Curvature-sensitive trans-assembly of human Atg8-family proteins in autophagy-related membrane tethering.Protein science : a publication of the Protein Society · 2020Article
- Influence of Long-Chain/Medium-Chain Triglycerides and Whey Protein/Tween 80 Ratio on the Stability of Phosphatidylserine Emulsions (O/W).ACS omega · 2020Article
- Sec17 (α-SNAP) and Sec18 (NSF) restrict membrane fusion to R-SNAREs, Q-SNAREs, and SM proteins from identical compartments.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Securing the Payload, Finding the Cell, and Avoiding the Endosome: Peptide-Targeted, Fusogenic Porous Silicon Nanoparticles for Delivery of siRNA.Advanced materials (Deerfield Beach, Fla.) · 2019Article
- Homotypic and heterotypicThe Journal of biological chemistry · 2019Article
- Immunogene therapy with fusogenic nanoparticles modulates macrophage response to Staphylococcus aureus.Nature communications · 2018Article
- Sequential actions of phosphatidylinositol phosphates regulate phagosome-lysosome fusion.Molecular biology of the cell · 2018Article
- Assembly of intermediates for rapid membrane fusion.The Journal of biological chemistry · 2018Article
- A cascade of multiple proteins and lipids catalyzes membrane fusion.Molecular biology of the cell · 2017Review
- High Transmembrane Voltage Raised by Close Contact Initiates Fusion Pore.Frontiers in molecular neuroscience · 2016Article
- A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly.Science (New York, N.Y.) · 2015Article
- SNAREs support atlastin-mediated homotypic ER fusion in Saccharomyces cerevisiae.The Journal of cell biology · 2015Article
- Platelet membrane variations and their effects on δ-granule secretion kinetics and aggregation spreading among different species.Biochimica et biophysica acta · 2015Article
- Towards reconstitution of membrane fusion mediated by SNAREs and other synaptic proteins.Critical reviews in biochemistry and molecular biology · 2015Review
- Differential Effects of Munc18s on Multiple Degranulation-Relevant Trans-SNARE Complexes.PloS one · 2015Article
- Phosphatidylserine in the brain: metabolism and function.Progress in lipid research · 2014Review
18 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
At low surface concentrations that permit formation of impermeable membranes, neuronal soluble N-ethyl maleimide sensitive factor attachment protein receptor (SNARE) proteins form a stable, parallel, trans complex when vesicles are brought into contact by a low concentration of poly(ethylene glycol) (PEG). Surprisingly, formation of a stable SNARE complex does not trigger fusion under these conditions. However, neuronal SNAREs do promote fusion at low protein/lipid ratios when triggered by higher concentrations of PEG. Promotion of PEG-triggered fusion required phosphatidylserine and depended only on the surface concentration of SNAREs and not on the formation of a trans SNARE complex. These results were obtained at protein surface concentrations reported for synaptobrevin in synaptic vesicles and with an optimally fusogenic lipid composition. At a much higher protein/lipid ratio, vesicles joined by SNARE complex slowly mixed lipids at 37 degrees C in the absence of PEG, in agreement with earlier reports. However, vesicles containing syntaxin at a high protein/lipid ratio (>or=1:250) lost membrane integrity. We conclude that the neuronal SNARE complex promotes fusion by joining membranes and that the individual proteins syntaxin and synaptobrevin disrupt membranes so as to favor formation of a stalk complex and to promote conversion of the stalk to a fusion pore. These effects are similar to the effects of viral fusion peptides and transmembrane domains, but they are not sufficient by themselves to produce fusion in our in vitro system at surface concentrations documented to occur in synaptic vesicles. Thus, it is likely that proteins or factors other than the SNARE complex must trigger fusion in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.