ArticlePlant physiology2005
Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 186 papers.
What it found
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Who cites it
186 citing papers in PubMed, 391 citations in OpenAlex.
- Article
- Comparative analysis of Cdc48-dependent proteolysis at the ER, mitochondria and chloroplasts.Nature communications · 2026Review
- Characterization and expression analysis of UBC gene family provide insights into the potential roles in pigment biosynthesis in pepper fruit.BMC genomics · 2026Article
- Ligand-induced ubiquitination regulates endocytosis and homeostasis of the ERECTA receptor kinase for stomatal development.The New phytologist · 2026Article
- Genome-wide identification and expression analysis of RBR-type ubiquitin ligase family genes reveal the role of ZmIBR10 in maize disease resistance.BMC plant biology · 2026Article
- The Hrd1-Mediated ERAD Pathway in Plants: Conserved Principles and Plant-Specific Innovations.International journal of molecular sciences · 2026Review
- An In Vitro Ubiquitination Assay to Study Phytoplasmal Effector Function.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Molecular Phylogeny of the SELMA Translocation Machinery Recounts the Evolution of Complex Photosynthetic Eukaryotes.Molecular biology and evolution · 2025Article
- The StUBC18-StPUB40 pair negatively regulate drought stress tolerance and influences tuber yield in potato.Horticulture research · 2025Article
- Genome-wide identification of wheat Ubiquitin-conjugating enzyme gene family and its role in wheat powdery mildew disease resistance.BMC plant biology · 2025Article
- Mapping of resistance genes to powdery mildew based on DNA re-sequencing and bulk segregant analysis in Capsicum.Protoplasma · 2025Article
- Plant ubiquitin E2 enzymes UBC32, UBC33, and UBC34 are involved in ERAD and function in host stress tolerance.BMC plant biology · 2025Article
- Quantitative proteomics reveals extensive lysine ubiquitination and transcription factor stability states in Arabidopsis.The Plant cell · 2024Article
- Ubiquitin E3 ligases in the plant Arg/N-degron pathway.The Biochemical journal · 2024Review
- StUBC13, a Ubiquitin-Conjugating Enzyme, Positively Regulates Salt and Osmotic Stresses in Potato.International journal of molecular sciences · 2024Article
- Genome-Wide Identification of theInternational journal of molecular sciences · 2024Article
- Genome‑wide identification and expression analysis of the UBC gene family in wheat (Triticum aestivum L.).BMC plant biology · 2024Article
- The CUL3A-LFH1-UBC15 ubiquitin ligase complex mediates SHORT VEGETATIVE PHASE degradation to accelerate flowering at high ambient temperature.Plant communications · 2024Article
- A dynamic ubiquitination balance of cell proliferation and endoreduplication regulators determines plant organ size.Science advances · 2024Article
- A transposon insertion in the promoter of OsUBC12 enhances cold tolerance during japonica rice germination.Nature communications · 2024Article
126 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Attachment of ubiquitin to substrate proteins is catalyzed by the three enzymes E1, E2 (ubiquitin conjugating [UBC]), and E3 (ubiquitin ligase). Forty-one functional proteins with a UBC domain and active-site cysteine are predicted in the Arabidopsis (Arabidopsis thaliana) genome, which includes four that are predicted or shown to function with ubiquitin-like proteins. Only nine were previously characterized biochemically as ubiquitin E2s. We obtained soluble protein for 22 of the 28 uncharacterized UBCs after expression in Escherichia coli and demonstrated that 16 function as ubiquitin E2s. Twelve, plus three previously characterized ubiquitin E2s, were also tested for the ability to catalyze ubiquitination in vitro in the presence of one of 65 really interesting new gene (RING) E3 ligases. UBC22, UBC19-20, and UBC1-6 had variable levels of E3-independent activity. Six UBCs were inactive with all RINGs tested. Closely related UBC8, 10, 11, and 28 were active with the largest number of RING E3s and with all RING types. Expression analysis was performed to determine whether E2s or E3s were expressed in specific organs or under specific environmental conditions. Closely related E2s show unique patterns of expression and most express ubiquitously. Some RING E3s are also ubiquitously expressed; however, others show organ-specific expression. Of all the organs tested, RING mRNAs are most abundant in floral organs. This study demonstrates that E2 diversity includes examples with broad and narrow specificity toward RINGs, and that most ubiquitin E2s are broadly expressed with each having a unique spatial and developmental pattern of expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.