Evidence map›Paper›PMID 16339805›Full record

ArticlePlant physiology2005

Use of two-color fluorescence-tagged transgenes to study interphase chromosomes in living plants.

Antonius J M Matzke, Bruno Huettel, Johannes van der Winden, Marjori Matzke

Open access · greenAbstract read
In one paragraph

Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
3.7field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 51 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Article
  5. Article
  6. Application and prospects of CRISPR/Cas9-based methods to trace defined genomic sequences in living and fixed plant cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020
    Review
  7. Article
  8. Global impacts of chromosomal imbalance on gene expression inProceedings of the National Academy of Sciences of the United States of America · 2018
    Article
  9. Article
  10. Genomes in Focus: Development and Applications of CRISPR-Cas9 Imaging Technologies.Angewandte Chemie (International ed. in English) · 2018
    Review
  11. Article
  12. Article
  13. Imaging Specific Genomic DNA in Living Cells.Annual review of biophysics · 2016
    Review
  14. Review
  15. Review
  16. Engineering of plant chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015
    Review
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Antonius J M MatzkeGregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Dr. Bohr-Gasse 3, A-1030 Vienna, Austria. antonius.matzke@gmi.oeaw.ac.at
Bruno Huettel
Johannes van der Winden
Marjori Matzke
Gregor Mendel Institute of Molecular Plant Biology · ATAustrian Academy of Sciences · AT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sixteen distinct sites distributed on all five Arabidopsis (Arabidopsis thaliana) chromosomes have been tagged using different fluorescent proteins and one of two different bacterial operator-repressor systems: (1) a yellow fluorescent protein-Tet repressor fusion protein bound to tet operator sequences, or (2) a green or red fluorescent protein-Lac repressor fusion protein bound to lac operator sequences. Individual homozygous lines and progeny of intercrosses between lines have been used to study various aspects of interphase chromosome organization in root cells of living, untreated seedlings. Features reported here include distances between transgene alleles, distances between transgene inserts on different chromosomes, distances between transgene inserts on the same chromatin fiber, alignment of homologous chromosomes, and chromatin movement. The overall findings are consistent with a random and largely static arrangement of interphase chromosomes in nuclei of root cells. These transgenic lines provide tools for in-depth analyses of interphase chromosome organization, expression, and dynamics in living plants.

Indexed as

AllelesArabidopsisBacterial ProteinsBase SequenceChromosomes, PlantDNA, BacterialFluorescent DyesGenetic TechniquesGreen Fluorescent ProteinsInterphaseLuminescent ProteinsMicroscopy, FluorescencePlants, Genetically ModifiedRecombinant Fusion ProteinsRed Fluorescent ProteinBacterial ProteinsDNA, BacterialFluorescent DyesGreen Fluorescent ProteinsLuminescent ProteinsRecombinant Fusion ProteinsRed Fluorescent ProteinT-DNAyellow fluorescent protein, Bacteria

Identifiers

PMID16339805
PMCPMC1310544
OpenAlexW2109380318

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.