ArticleProceedings of the National Academy of Sciences of the United States of America2005
RHL1 is an essential component of the plant DNA topoisomerase VI complex and is required for ploidy-dependent cell growth.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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50 citing papers in PubMed, 123 citations in OpenAlex.
- Molecular regulation of plant endoreduplication: core mechanisms and their upstream control.Plant molecular biology · 2026Review
- A simple method to efficiently generate structural variation in plants.PLoS genetics · 2025Article
- The endocycle regulators SIM and CCS52A1 control Arabidopsis root hair cell ploidy and expansion.Plant physiology · 2025Article
- The CIN-TCP transcription factors regulate endocycle progression and pavement cell size by promoting cell wall pectin degradation.Nature communications · 2025Article
- Generation of viable hypomorphic and null mutant plants via CRISPR-Cas9 targeting mRNA splicing sites.Journal of plant research · 2025Article
- The plant early recombinosome: a high security complex to break DNA during meiosis.Plant reproduction · 2024Review
- Phylogenetic distribution of DNA topoisomerase VI and its distinction from SPO11.NAR genomics and bioinformatics · 2024Article
- Chromatin dynamics and RNA metabolism are double-edged swords for the maintenance of plant genome integrity.Nature plants · 2024Review
- Physiology, genomics, and evolutionary aspects of desert plants.Journal of advanced research · 2024Review
- Genome-wide association study for yield-related traits in faba bean (Frontiers in plant science · 2024Article
- How Do Plants Cope with DNA Damage? A Concise Review on the DDR Pathway in Plants.International journal of molecular sciences · 2023Review
- Evolution and Diversity of the TopoVI and TopoVI-like Subunits With Extensive Divergence of the TOPOVIBL subunit.Molecular biology and evolution · 2022Article
- Topoisomerase VI participates in an insulator-like function that prevents H3K9me2 spreading.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Arabidopsis thaliana subclass I ACTIN DEPOLYMERIZING FACTORs and vegetative ACTIN2/8 are novel regulators of endoreplication.Journal of plant research · 2021Article
- DNA topoisomerases: Advances in understanding of cellular roles and multi-protein complexes via structure-function analysis.BioEssays : news and reviews in molecular, cellular and developmental biology · 2021Review
- The AGCVIII kinase Dw2 modulates cell proliferation, endomembrane trafficking, and MLG/xylan cell wall localization in elongating stem internodes of Sorghum bicolor.The Plant journal : for cell and molecular biology · 2021Article
- A Spatiotemporal DNA Endoploidy Map of the Arabidopsis Root Reveals Roles for the Endocycle in Root Development and Stress Adaptation.The Plant cell · 2018Article
- Topoisomerase VI senses and exploits both DNA crossings and bends to facilitate strand passage.eLife · 2018Article
- GTL1 and DF1 regulate root hair growth through transcriptional repression ofDevelopment (Cambridge, England) · 2018Article
- Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
How cells achieve their final sizes is a pervasive biological question. One strategy to increase cell size is for the cell to amplify its chromosomal DNA content through endoreduplication cycles. Although endoreduplication is widespread in eukaryotes, we know very little about its molecular mechanisms. Successful progression of the endoreduplication cycle in Arabidopsis requires a plant homologue of archaeal DNA topoisomerase (topo) VI. To further understand how DNA is endoreduplicated and how this process is regulated, we isolated a dwarf Arabidopsis mutant, hyp7 (hypocotyl 7), in which various large cell types that in the wild type normally endoreduplicate multiple times complete only the first two rounds of endoreduplication and stall at 8C. HYP7 encodes the RHL1 (ROOT HAIRLESS 1) protein, and sequence analysis reveals that RHL1 has similarity to the C-terminal domain of mammalian DNA topo IIalpha, another type II topo that shares little sequence homology with topo VI. RHL1 shows DNA binding activity in vitro, and we present both genetic and in vivo evidence that RHL1 forms a multiprotein complex with plant topo VI. We propose that RHL1 plays an essential role in the topo VI complex to modulate its function and that the two distantly related topos, topo II and topo VI, have evolved a common domain that extends their function. Our data suggest that plant topo II and topo VI play distinct but overlapping roles during the mitotic cell cycle and endoreduplication cycle.
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