ArticleClinical and diagnostic laboratory immunology2005
Immunoglobulin G1 enzyme-linked immunosorbent assay for diagnosis of Johne's Disease in red deer (Cervus elaphus).
Article in Clinical and diagnostic laboratory immunology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 53 citations in OpenAlex.
- Evaluation of three ELISA assays for detection of Mycobacterium avium subsp. paratuberculosis antibodies in serum of sika deer (Cervus nippon).Scientific reports · 2026Article
- Development of a Method to DetectFrontiers in veterinary science · 2021Article
- Developments in the HCV Screening Technologies Based on the Detection of Antigens and Antibodies.Sensors (Basel, Switzerland) · 2019Review
- Control of Mycobacterium avium subsp. paratuberculosis infection on a New Zealand pastoral dairy farm.BMC veterinary research · 2019Article
- The effect of sub-clinical infection with Mycobacterium avium subsp. paratuberculosis on milk production in a New Zealand dairy herd.BMC veterinary research · 2018Article
- Control of bovine tuberculosis in a farmed red deer herd in England.The Veterinary record · 2017Article
- Effects of repeated comparative intradermal tuberculin testing on test results: a longitudinal study in TB-free red deer.BMC veterinary research · 2016Article
- Epidemiology, diagnostics, and management of tuberculosis in domestic cattle and deer in New Zealand in the face of a wildlife reservoir.New Zealand veterinary journal · 2015Review
- Sensitive and specific enzyme-linked immunosorbent assay for detecting serum antibodies against Mycobacterium avium subsp. paratuberculosis in fallow deer.Clinical and vaccine immunology : CVI · 2014Article
- Farmed deer: A veterinary model for chronic mycobacterial diseases that is accessible, appropriate and cost-effective.Journal of pharmacy & bioallied sciences · 2014Review
- Composite testing for ante-mortem diagnosis of Johne's disease in farmed New Zealand deer: correlations between bacteriological culture, histopathology, serological reactivity and faecal shedding as determined by quantitative PCR.BMC veterinary research · 2013Article
- Innate immune markers that distinguish red deer (Cervus elaphus) selected for resistant or susceptible genotypes for Johne's disease.Veterinary research · 2013Article
- The modification and evaluation of an ELISA test for the surveillance of Mycobacterium avium subsp. paratuberculosis infection in wild ruminants.BMC veterinary research · 2013Article
- Longitudinal Pathogenesis Study of Young Red Deer (Cervus elaphus) after Experimental Challenge with Mycobacterium avium subsp. paratuberculosis (MAP).Veterinary medicine international · 2012Article
- Immunoregulatory cytokines are associated with protection from immunopathology following Mycobacterium avium subspecies paratuberculosis infection in red deer.Infection and immunity · 2011Article
- Identification of immune parameters to differentiate disease states among sheep infected with Mycobacterium avium subsp. paratuberculosis.Clinical and vaccine immunology : CVI · 2010Article
- Diagnostic detection methods for Mycobacterium avium subsp. paratuberculosis in white-tailed deer.The Canadian veterinary journal = La revue veterinaire canadienne · 2008Article
- Differential immune responses of red Deer (Cervus elaphus) following experimental challenge with Mycobacterium avium subsp. paratuberculosis.Clinical and vaccine immunology : CVI · 2008Article
- Proper estimation of sensitivity and specificity.Clinical and vaccine immunology : CVI · 2006Article
- Isolation of high-affinity single-chain antibodies against Mycobacterium avium subsp. paratuberculosis surface proteins from sheep with Johne's disease.Clinical and vaccine immunology : CVI · 2006Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study was designed to develop a customized enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of Johne's disease (JD) in farmed deer. Two antigens were selected on the basis of their superior diagnostic readouts: denatured purified protein derivative (PPDj) and undenatured protoplasmic antigen (PpAg). ELISA development was based on the antigen reactivity of the immunoglobulin G1 (IgG1) isotype, which is a highly specific marker for mycobacterial disease seroreactivity in deer. Sensitivity estimates and test parameters were established using 102 Mycobacterium paratuberculosis-infected animals from more than 10 deer herds, and specificity estimates were determined using 508 uninfected animals from 5 known disease-free herds. A receiver-operated characteristic analysis determined that at a cut point of 50 ELISA units, there was a specificity of 99.5% and sensitivities of 84.0% with PPDj antigen, 88.0% with PpAg, and 91.0% when the antigens were used serially in a composite test. Estimated sensitivity was further improved using recombinant protein antigens unique for M. paratuberculosis, which identified infected animals that were unreactive to PPDj or PpAg. While 80% of animals that were seropositive in the IgG1 ELISA had detectable histopathology, the assay could also detect animals with subclinical disease. The test was significantly less sensitive (75%) for animals that were culture positive for M. paratuberculosis but with no detectable pathology than for those with pathological evidence of JD (>90%). When the IgG1 ELISA was used annually over a 4-year period in a deer herd with high levels of clinical JD, it eliminated clinical disease, increased production levels, and reduced JD-related mortality.
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