Evidence map›Paper›PMID 16335802›Full record

ArticleMolecular and cellular biochemistry2005

Tryptophan residue is essential for immunoreactivity of a diagnostically relevant peptide epitope of A. fumigatus.

Neel Kamal, Shantanu Chowdhury, Taruna Madan, Deepak Sharma, M Attreyi, Wahajul Haq, Seturam Bandacharya Katti, Anil Kumar, P Usha Sarma

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Molecular and cellular biochemistry, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Neel KamalInstitute of Genomics and Integrative Biology, Mall Road, Delhi, India.
Shantanu Chowdhury
Taruna Madan
Deepak Sharma
M Attreyi
Wahajul Haq
Seturam Bandacharya Katti
Anil Kumar
P Usha Sarma
Institute of Genomics and Integrative Biology · INCentral Drug Research Institute · INUniversity of Delhi · INDevi Ahilya Vishwavidyalaya · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The role of tryptophan (Trp17) in immunoreactivity of P1, the diagnostically relevant peptide from a major allergen/antigen of Aspergillus fumigatus, was evaluated by chemically modifying tryptophanyl residue of P1. In BIAcore kinetic studies, unmodified P1 showed a 100-fold higher binding with ABPA (Allergic Bronchopulmonary Aspergillosis) patients' IgG [KD (equilibrium dissociation constant) = 2.74 e(-8) +/- 0.13 M] than the controls' IgG (KD = 2.97 e(-6) +/- 0.14 M), whereas chemically-modified P1 showed similar binding [KD patients' IgG = 3.25 e(-7) +/- 0.16 M, KD controls' IgG = 3.86 e(-7) +/- 0.19 M] indicating loss of specific immunoreactivity of P1 on tryptophan modification. Modified P1 showed loss of specific binding to IgE and IgG antibodies of ABPA patients in ELISA (Enzyme-Linked Immunosorbent Assay). The study infers that tryptophan residue (Trp17) is essential for immunoreactivity of P1.

Indexed as

EpitopesAspergillosis, Allergic BronchopulmonaryAspergillus fumigatusBioreactorsCase-Control StudiesCircular DichroismComputer SimulationCrystallography, X-RayEnzyme-Linked Immunosorbent AssayHumansHydrogen-Ion ConcentrationImmunoglobulin EImmunoglobulin GKineticsModels, MolecularPeptidesEpitopesImmunoglobulin EImmunoglobulin GPeptidesSolventsTrifluoroethanolTryptophan

Identifiers

PMID16335802
OpenAlexW1982711665

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.