ArticleMolecular and cellular biochemistry2005
Effect of melatonin on phagocytic activity and intracellular free calcium concentration in testicular macrophages from normal and streptozotocin-induced diabetic rats.
Article in Molecular and cellular biochemistry, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 36 citations in OpenAlex.
- Pineal Gland and Melatonin are Associated with Serum Element Metabolism in Rats.Noro psikiyatri arsivi · 2025Article
- Melatonin shapes bacterial clearance function of porcine macrophages during enterotoxigenicAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Article
- Coenzyme Q10 and Melatonin for the Treatment of Male Infertility: A Narrative Review.Nutrients · 2022Review
- Melatonin Hormone Acts on Cells of Maternal Blood and Placenta From Diabetic Mothers.Frontiers in physiology · 2021Article
- Melatonin Ameliorates Inflammation and Oxidative Stress by Suppressing the p38MAPK Signaling Pathway in LPS-Induced Sheep Orchitis.Antioxidants (Basel, Switzerland) · 2020Article
- Local Actions of Melatonin in Somatic Cells of the Testis.International journal of molecular sciences · 2017Review
- Influence of melatonin on the immune system of fish: a review.International journal of molecular sciences · 2013Review
- Antioxidant effect of melatonin on the functional activity of colostral phagocytes in diabetic women.PloS one · 2013Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study examined the effect of melatonin (MLT) on in vitro phagocytosis of testicular macrophages taken from control and streptozotocin (STZ)-induced diabetic rats and the possible mechanism of its action. The phagocytic activity was measured as a number of latex beads ingested by 100 macrophages (PI, phagocytic index) in consecutive time points of the incubation. Changes in intracellular free calcium level [Ca2+]i in isolated macrophages in vitro were measured with the use of ratio-image fluorescence microscopy (fluorescent dye: Fura2/AM). Phagocytic index in macrophages isolated from healthy rats was 20% higher than in those from diabetic animals. Melatonin in physiological concentration (10(-7) M) significantly (p < 0.05) increased the PI in testicular macrophages from control animals (PI = 68 +/- 5 with MLT compared to PI = 46 +/- 7 without MLT) while no such effect was observed in the cells from diabetic rats (PI = 36 +/- 23 with MLT compared to PI = 31 +/- 11 without MLT). Basal [Ca2+]i was significantly (p < 0.01) higher in macrophages from diabetic rats compared to control. Stimulation of both control and diabetic testicular macrophages with 10(-7) M MLT resulted in a significant (p < 0.05) increase in [Ca2+]i in cells incubated in 2.5 mM calcium solution while no such response was observed in calcium-free Tyrode solution. However, MLT evoked [Ca2+]i response in macrophages isolated from diabetic animals was much lower than in macrophages isolated from age-matched controls and the time needed for maximal response was much longer. Lack of response in calcium-free solution suggests that extracellular calcium may be necessary to trigger MLT response and in its progression.
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