ArticleJournal of clinical microbiology2005
Rapid determination of rifampin resistance in clinical isolates of Mycobacterium tuberculosis by real-time PCR.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- Clinical Evaluation of a Tandem qPCR-CRISPR Assay for Multidrug-Resistant or Rifampicin-Resistant Tuberculosis: A Prospective Diagnostic Accuracy Study.Microorganisms · 2026Article
- Rifampicin Mono-Resistant Tuberculosis-A Review of an Uncommon But Growing Challenge for Global Tuberculosis Control.Open forum infectious diseases · 2021Review
- Distribution and Frequency ofInfection and drug resistance · 2020Article
- Rapid Detection of Rifampicin- and Isoniazid-ResistantJundishapur journal of microbiology · 2016Article
- Study of the rifampin monoresistance mechanism in Mycobacterium tuberculosis.Antimicrobial agents and chemotherapy · 2013Article
- Fast test for assessing the susceptibility of Mycobacterium tuberculosis to isoniazid and rifampin by real-time PCR.Memorias do Instituto Oswaldo Cruz · 2012Article
- Rapid, high-throughput detection of rifampin resistance and heteroresistance in Mycobacterium tuberculosis by use of sloppy molecular beacon melting temperature coding.Journal of clinical microbiology · 2012Article
- Multiplex real-time PCR melting curve assay to detect drug-resistant mutations of Mycobacterium tuberculosis.Journal of clinical microbiology · 2011Article
- High-resolution melting curve analysis for rapid detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis clinical isolates.Journal of clinical microbiology · 2010Article
- A Kinetic Study of In Vitro Lysis of Mycobacterium smegmatis.Chemical engineering science · 2009Article
- Performance of the Genotype MTBDRPlus assay in the diagnosis of tuberculosis and drug resistance in Samara, Russian Federation.BMC clinical pathology · 2009Article
- rpoB Gene mutations and molecular characterization of rifampin-resistant Mycobacterium tuberculosis isolates from Shandong Province, China.Journal of clinical microbiology · 2006Article
- Evaluation of the TB-Biochip oligonucleotide microarray system for rapid detection of rifampin resistance in Mycobacterium tuberculosis.Journal of clinical microbiology · 2006Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Real-time PCR was used to determine rifampin resistance in clinical isolates of Mycobacterium tuberculosis. Ninety-six rifampin-resistant isolates and 23 rifampin-susceptible isolates were included in the study. A 305-bp region covering the 81-bp "rifampin resistance-determining region" of rpoB was amplified. Two hybridization probe pairs that covered the most frequent mutation sites in rpoB, codon regions 526 to 531 and 513 to 516, were used. The results obtained by real-time PCR were compared to those obtained by the proportion method. For detection of rifampin resistance, the real-time PCR assay yielded a sensitivity of 92.7% and a specificity of 100%. Real-time PCR is a very rapid method, and it can be especially helpful for the reporting of resistant clinical isolates in a very short period of time.
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