ArticleJournal of clinical microbiology2005
Identification of 43 Streptococcus species by pyrosequencing analysis of the rnpB gene.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Microbiome in radiotherapy: an emerging approach to enhance treatment efficacy and reduce tissue injury.Molecular medicine (Cambridge, Mass.) · 2024Review
- Evaluation of the correctable decoding sequencing as a new powerful strategy for DNA sequencing.Life science alliance · 2022Article
- An Overview onInternational journal of molecular sciences · 2019Review
- Synergism of Plant Compound With Traditional Antimicrobials AgainstFrontiers in microbiology · 2018Article
- The Potential Effect of Oral Microbiota in the Prediction of Mucositis During Radiotherapy for Nasopharyngeal Carcinoma.EBioMedicine · 2017Article
- Improved Differentiation of Streptococcus pneumoniae and Other S. mitis Group Streptococci by MALDI Biotyper Using an Improved MALDI Biotyper Database Content and a Novel Result Interpretation Algorithm.Journal of clinical microbiology · 2017Article
- A Genome-Wide Profiling Strategy as an Aid for Searching Unique Identification Biomarkers for Streptococcus.Indian journal of microbiology · 2016Article
- Differentiation and phylogenetic relationships in Mycobacterium spp with special reference to the RNase P RNA gene rnpB.Current microbiology · 2014Article
- Antibiotic susceptibilities, streptococcal pyrogenic exotoxin gene profiles among clinical isolates of group C or G Streptococcus dysgalactiae subsp. equisimilis & of group G S. anginosus group at a tertiary care centre.The Indian journal of medical research · 2014Article
- Molecular diagnosis of sepsis: New aspects and recent developments.European journal of microbiology & immunology · 2014Article
- Article
- Investigation of supragingival plaque microbiota in different caries status of Chinese preschool children by denaturing gradient gel electrophoresis.Microbial ecology · 2011Article
- Identification of clinically relevant viridans group streptococci by phenotypic and genotypic analysis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011Article
- It's not easy being green: the viridans group streptococci, with a focus on pediatric clinical manifestations.Journal of clinical microbiology · 2010Review
- Extended Safety Data for the Oral Cavity Probiotic Streptococcus salivarius K12.Probiotics and antimicrobial proteins · 2010Article
- Development of real-time PCR assays for detection of the Streptococcus milleri group from cystic fibrosis clinical specimens by targeting the cpn60 and 16S rRNA genes.Journal of clinical microbiology · 2010Article
- Infective Endocarditis: Identification of Catalase-Negative, Gram-Positive Cocci from Blood Cultures by Partial 16S rRNA Gene Analysis and by Vitek 2 Examination.The open microbiology journal · 2010Article
- Utility of pyrosequencing in identifying bacteria directly from positive blood culture bottles.Journal of clinical microbiology · 2009Article
- Pyrosequencing analysis of the gyrB gene to differentiate bacteria responsible for diarrheal diseases.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2008Article
- DNA pyrosequencing-based bacterial pathogen identification in a pediatric hospital setting.Journal of clinical microbiology · 2007Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pyrosequencing technology was evaluated for identification of species within the Streptococcus genus. Two variable regions in the rnpB gene, which encodes the RNA subunit of endonuclease P, were sequenced in two reactions. Of 43 species, all could be identified to the species level except strains of the species pairs Streptococcus anginosus/S. constellatus and S. infantis/S. peroris. A total of 113 blood culture isolates were identified by pyrosequencing analysis of partial rnpB sequences. All but eight isolates could be unambiguously assigned to a specific species when the first 30 nucleotides of the two regions were compared to an rnpB database comprising 107 streptococcal strains. Principal coordinate analysis of sequence variation of strains from viridans group streptococci resulted in species-specific clusters for the mitis and the salivarius groups but not for the anginosus group. The identification capacity of pyrosequencing was compared to the biochemical test systems VITEK 2 and Rapid ID 32 Strep. The concordance between pyrosequencing and VITEK 2 was 75%, and for Rapid ID 32 Strep the corresponding figure was 77%. Isolates with discrepant identifications in the three methods were subjected to entire rnpB DNA sequence analysis that confirmed the identifications by pyrosequencing. In conclusion, pyrosequencing analysis of the rnpB gene can reliably identify Streptococcus species with high resolution.
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