ArticleApplied and environmental microbiology2005
Identification and localization of extraradicular biofilm-forming bacteria associated with refractory endodontic pathogens.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 106 citations in OpenAlex.
- Antibacterial efficacy of intracanal medicaments on bacterial biofilm: a critical review.Journal of applied oral science : revista FOBPooled it
- Efficacy of Smear Layer Removal at the Apical One-Third of the Root Using Different Protocols of Erbium-Doped Yttrium Aluminium Garnet (Er:YAG) Laser.Medicina (Kaunas, Lithuania) · 2023Trial
- Multi-modular bone healing assessment in a randomized controlled clinical trial of root-end surgery with the use of leukocyte- and platelet-rich fibrin and an occlusive membrane.Clinical oral investigations · 2020Trial
- Microbiome of Apical Intracanal and Extraradicular Biofilms From the Same Roots of Teeth With Persistent Apical Periodontitis: An Observational Study.International endodontic journal · 2026Observational
- Is apical periodontitis a matter of microbial diversity or time? A scoping review.Head & face medicine · 2026Article
- Review
- Efficacy of Antimicrobial Peptide GH12 on a Multispecies Endodontic Biofilm Model: An In-vitro Study.European endodontic journal · 2024Article
- Article
- Evidence on the Use of Mouthwash for the Control of Supragingival Biofilm and Its Potential Adverse Effects.Antibiotics (Basel, Switzerland) · 2022Review
- Plasma Rich in Growth Factors in the Treatment of Endodontic Periapical Lesions in Adult Patients: A Narrative Review.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Microbial Communities in the Extraradicular and Intraradicular Infections Associated With Persistent Apical Periodontitis.Frontiers in cellular and infection microbiology · 2021Article
- A qualitative and semiquantitative SEM study of the morphology of the biofilm on root surfaces of human teeth with endodontic-periodontal lesions.Experimental and therapeutic medicine · 2020Article
- Amoebae in Chronic, Polymicrobial Endodontic Infections Are Associated with Altered Microbial Communities of Increased Virulence.Journal of clinical medicine · 2020Article
- Disturbed bone remodelling activity varies in different stages of experimental, gradually progressive apical periodontitis in rats.International journal of oral science · 2019Article
- Article
- Apical periodontitis associated with a calculus-like deposit: A case report of a rare fan-shaped manifestation.Annals of medicine and surgery (2012) · 2019Article
- Influence of microbiology on endodontic failure. Literature review.Medicina oral, patologia oral y cirugia bucal · 2019Review
- Epithelioid cell granuloma with caseating necrosis possibly caused by periapical periodontitis: a case report.Journal of medical case reports · 2018Article
- Factors that cause endodontic failures in general practices in Japan.BMC oral health · 2018Observational
- Enzyme-labeled Antigen Method: Development and Application of the Novel Approach for Identifying Plasma Cells Locally Producing Disease-specific Antibodies in Inflammatory Lesions.Acta histochemica et cytochemica · 2016Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial biofilms have been found to develop on root surfaces outside the apical foramen and be associated with refractory periapical periodontitis. However, it is unknown which bacterial species form extraradicular biofilms. The present study aimed to investigate the identity and localization of bacteria in human extraradicular biofilms. Twenty extraradicular biofilms, used to identify bacteria using a PCR-based 16S rRNA gene assay, and seven root-tips, used to observe immunohistochemical localization of three selected bacterial species, were taken from 27 patients with refractory periapical periodontitis. Bacterial DNA was detected from 14 of the 20 samples, and 113 bacterial species were isolated. Fusobacterium nucleatum (14 of 14), Porphyromonas gingivalis (12 of 14), and Tannellera forsythensis (8 of 14) were frequently detected. Unidentified and uncultured bacterial DNA was also detected in 11 of the 14 samples in which DNA was detected. In the biofilms, P. gingivalis was immunohistochemically detected in all parts of the extraradicular biofilms. Positive reactions to anti-F. nucleatum and anti-T. forsythensis sera were found at specific portions of the biofilm. These findings suggested that P. gingivalis, T. forsythensis, and F. nucleatum were associated with extraradicular biofilm formation and refractory periapical periodontitis.
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Registered trials
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