ArticleApplied and environmental microbiology2005
Characterization of microbial communities found in the human vagina by analysis of terminal restriction fragment length polymorphisms of 16S rRNA genes.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Safety assessment scheme for menstrual cups and application for the evaluation of a menstrual cup comprised of medical grade silicone.EBioMedicine · 2022Trial
- Trial
- Characterization of the vaginal microbiome and its metabolic potential in Colombian patients with recurrent vulvovaginal candidiasis.Medical mycology · 2026Article
- Potential applications and mechanisms of natural products in mucosal-related diseases.Frontiers in immunology · 2025Review
- Birth Mode Does Not Determine the Presence of Shared Bacterial Strains between the Maternal Vaginal Microbiome and the Infant Stool Microbiome.Microbiology spectrum · 2023Article
- The urothelium: a multi-faceted barrier against a harsh environment.Mucosal immunology · 2022Review
- Analysis of Microbial Communities: An Emerging Tool in Forensic Sciences.Diagnostics (Basel, Switzerland) · 2021Review
- The Human Vulvar Microbiome: A Systematic Review.Microorganisms · 2021Review
- The Vaginal Microbiome: A Long Urogenital Colonization Throughout Woman Life.Frontiers in cellular and infection microbiology · 2021Review
- Genome-Wide Mutagenesis Identifies Factors Involved in Enterococcus faecalis Vaginal Adherence and Persistence.Infection and immunity · 2020Article
- Anadromous Arctic Char Microbiomes: Bioprospecting in the High Arctic.Frontiers in bioengineering and biotechnology · 2019Article
- The effect of vaginal microbial communities on colonization by Staphylococcus aureus with the gene for toxic shock syndrome toxin 1 (TSST-1): a case-control study.Pathogens and disease · 2018Article
- Article
- Microbial diversity in uterus of healthy and metritic postpartum Holstein dairy cows.Folia microbiologica · 2013Article
- The effects of a probiotic yeast on the bacterial diversity and population structure in the rumen of cattle.PloS one · 2013Article
- Article
- Development of a Lactobacillus specific T-RFLP method to determine lactobacilli diversity in complex samples.Journal of microbiological methods · 2012Article
- Understanding vaginal microbiome complexity from an ecological perspective.Translational research : the journal of laboratory and clinical medicine · 2012Review
- Molecular methods for pathogen and microbial community detection and characterization: current and potential application in diagnostic microbiology.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2012Review
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
To define and monitor the structure of microbial communities found in the human vagina, a cultivation-independent approach based on analyses of terminal restriction fragment length polymorphisms (T-RFLP) of 16S rRNA genes was developed and validated. Sixteen bacterial strains commonly found in the human vagina were used to construct model communities that were subsequently used to develop efficient means for the isolation of genomic DNA and an optimal strategy for T-RFLP analyses. The various genera in the model community could best be resolved by digesting amplicons made using bacterial primers 8f and 926r with HaeIII; fewer strains could be resolved using other primer-enzyme combinations, and no combination successfully distinguished certain species of the same genus. To demonstrate the utility of the approach, samples from five women that had been collected over a 2-month period were analyzed. Differences and similarities among the vaginal microbial communities of the women were readily apparent. The T-RFLP data suggest that the communities of three women were dominated by a single phylotype, most likely species of Lactobacillus. In contrast, the communities of two other women included numerically abundant populations that differed from Lactobacillus strains whose 16S rRNA genes had been previously determined. The T-RFLP profiles of samples from all the women were largely invariant over time, indicating that the kinds and abundances of the numerically dominant populations were relatively stable throughout two menstrual cycles. These findings show that T-RFLP of 16S rRNA genes can be used to compare vaginal microbial communities and gain information about the numerically dominant populations that are present.
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Registered trials
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