ArticleApplied and environmental microbiology2005
Enantioselective transformation of alpha-hexachlorocyclohexane by the dehydrochlorinases LinA1 and LinA2 from the soil bacterium Sphingomonas paucimobilis B90A.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Revelation of bioremediation approaches for hexachlorocyclohexane degradation in soil.World journal of microbiology & biotechnology · 2023Review
- Transformation of HBCDs by Rhodococcus sp. stu-38.Current microbiology · 2022Article
- Comparative proteomics unravelled the hexachlorocyclohexane (HCH) isomers specific responses in an archetypical HCH degrading bacterium Sphingobium indicum B90A.Environmental science and pollution research international · 2021Article
- Lake Superior Has Lost over 90% of Its Pesticide HCH Load since 1986.Environmental science & technology · 2021Article
- Seeking the Source of Catalytic Efficiency of Lindane Dehydrochlorinase, LinA.The journal of physical chemistry. B · 2020Article
- Genome Organization of Sphingobium indicum B90A: An Archetypal Hexachlorocyclohexane (HCH) Degrading Genotype.Genome biology and evolution · 2017Article
- Important amino acid residues of hexachlorocyclohexane dehydrochlorinases (LinA) for enantioselective transformation of hexachlorocyclohexane isomers.Biodegradation · 2017Article
- Enantioselective Resolution of (R,S)-Carvedilol to (S)-(-)-Carvedilol by Biocatalysts.Natural products and bioprospecting · 2017Article
- Biodegradability of HCH in agricultural soils from Guadeloupe (French West Indies): identification of the lin genes involved in the HCH degradation pathway.Environmental science and pollution research international · 2016Article
- Novel LinA type 3 δ-hexachlorocyclohexane dehydrochlorinase.Applied and environmental microbiology · 2015Article
- Insights into Ongoing Evolution of the Hexachlorocyclohexane Catabolic Pathway from Comparative Genomics of Ten Sphingomonadaceae Strains.G3 (Bethesda, Md.) · 2015Article
- Enantioselective dehydrochlorination of δ-Hexachlorocyclohexane and δ-Pentachlorocyclohexene by LinA1 and LinA2 from Sphingobium indicum B90A.Applied and environmental microbiology · 2013Article
- Genome sequence of Sphingobium indicum B90A, a hexachlorocyclohexane-degrading bacterium.Journal of bacteriology · 2012Article
- Comparative metagenomic analysis of soil microbial communities across three hexachlorocyclohexane contamination levels.PloS one · 2012Article
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- Evaluation of hexachlorocyclohexane contamination from the last lindane production plant operating in India.Environmental science and pollution research international · 2011Article
- Kinetic and sequence-structure-function analysis of known LinA variants with different hexachlorocyclohexane isomers.PloS one · 2011Article
- Biochemistry of microbial degradation of hexachlorocyclohexane and prospects for bioremediation.Microbiology and molecular biology reviews : MMBR · 2010Review
- Pseudomonas sp. to Sphingobium indicum: a journey of microbial degradation and bioremediation of Hexachlorocyclohexane.Indian journal of microbiology · 2008Article
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Abstract
Sphingomonas paucimobilis B90A contains two variants, LinA1 and LinA2, of a dehydrochlorinase that catalyzes the first and second steps in the metabolism of hexachlorocyclohexanes (R. Kumari, S. Subudhi, M. Suar, G. Dhingra, V. Raina, C. Dogra, S. Lal, J. R. van der Meer, C. Holliger, and R. Lal, Appl. Environ. Microbiol. 68:6021-6028, 2002). On the amino acid level, LinA1 and LinA2 were 88% identical to each other, and LinA2 was 100% identical to LinA of S. paucimobilis UT26. Incubation of chiral alpha-hexachlorocyclohexane (alpha-HCH) with Escherichia coli BL21 expressing functional LinA1 and LinA2 S-glutathione transferase fusion proteins showed that LinA1 preferentially converted the (+) enantiomer, whereas LinA2 preferred the (-) enantiomer. Concurrent formation and subsequent dissipation of beta-pentachlorocyclohexene enantiomers was also observed in these experiments, indicating that there was enantioselective formation and/or dissipation of these enantiomers. LinA1 preferentially formed (3S,4S,5R,6R)-1,3,4,5,6-pentachlorocyclohexene, and LinA2 preferentially formed (3R,4R,5S,6S)-1,3,4,5,6-pentachlorocyclohexene. Because enantioselectivity was not observed in incubations with whole cells of S. paucimobilis B90A, we concluded that LinA1 and LinA2 are equally active in this organism. The enantioselective transformation of chiral alpha-HCH by LinA1 and LinA2 provides the first evidence of the molecular basis for the changed enantiomer composition of alpha-HCH in many natural environments. Enantioselective degradation may be one of the key processes determining enantiomer composition, especially when strains that contain only one of the linA genes, such as S. paucimobilis UT26, prevail.
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